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Literature summary for 2.4.1.8 extracted from

  • Awad, F.; Kulinich, A.; Yao, M.; Duan, X.; Cai, Z.; Gu, B.; Liu, L.; Voglmeir, J.
    Enzymatic glycosylation of indoxyglycosides catalyzed by a novel maltose phosphorylase from Emticicia oligotrophica (2016), J. Carbohydr. Chem., 35, 301-314 .
No PubMed abstract available
Search for more literature for 2.4.1.8

Application

Application Comment Organism
synthesis simple expression and purification protocol and the use of maltose as an inexpensive starting material make this maltose phosphorylase from Emticicia oligotrophica a valuable biocatalyst for the synthesis of glucose-containing glycosides Emticicia oligotrophica

Cloned(Commentary)

Cloned (Comment) Organism
-
 Emticicia oligotrophica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 maltose pH 7.5, temperature not specified in the publication Emticicia oligotrophica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 SDS-PAGE Emticicia oligotrophica

Organism

Organism UniProt Comment Textmining
Emticicia oligotrophica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Emticicia oligotrophica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromo-4-chloro-3-indolyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + beta-D-glucose 1-phosphate
-
 Emticicia oligotrophica 5-bromo-4-chloro-3-indolyl 2-acetamido-2-deoxy-4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside + phosphate
-
 ?
5-bromo-4-chloro-3-indolyl alpha-D-glucopyranoside + beta-D-glucose 1-phosphate
-
 Emticicia oligotrophica 5-bromo-4-chloro-3-indolyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate
-
 ?
5-bromo-4-chloro-3-indolyl beta-D-glucopyranoside + beta-D-glucose 1-phosphate
-
 Emticicia oligotrophica 5-bromo-4-chloro-3-indolyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside + phosphate
-
 ?
D-glucose + beta-D-glucose 1-phosphate
-
 Emticicia oligotrophica maltose + phosphate
-
 r
maltose + phosphate high selectivity towards the phosphorolysis of maltose, whereas no phosphorolysis was observed using other glucose-containing disaccharides such as cellobiose, melibiose, sucrose and trehalose Emticicia oligotrophica D-glucose + beta-D-glucose 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
EoMP4038
-
 Emticicia oligotrophica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Emticicia oligotrophica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65 25°C: about 45% of maximal activity, 65°C: about 90% of maximal activity Emticicia oligotrophica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Emticicia oligotrophica

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 80% of maximal activity, pH 9.0: about 60% of maximal activity Emticicia oligotrophica