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Literature summary for 2.4.1.8 extracted from

  • Gao, Y.; Saburi, W.; Taguchi, Y.; Mori, H.
    Biochemical characteristics of maltose phosphorylase MalE from Bacillus sp. AHU2001 and chemoenzymatic synthesis of oligosaccharides by the enzyme (2019), Biosci. Biotechnol. Biochem., 83, 2097-2109 .
    View publication on PubMed
Search for more literature for 2.4.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Bacillus sp. AHU2001

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
 Bacillus sp. AHU2001

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.295
-
 phosphate pH 8.0, 37°C Bacillus sp. AHU2001
0.835
-
 maltose pH 8.0, 37°C Bacillus sp. AHU2001
5.68
-
 6-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
8.38
-
 D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
14.1
-
 1,5-anhydro-D-glucitol pH 8.0, 37°C Bacillus sp. AHU2001
15.1
-
 D-Lyxose pH 8.0, 37°C Bacillus sp. AHU2001
15.6
-
 D-glucosamine pH 8.0, 37°C Bacillus sp. AHU2001
16.3
-
 methyl alpha-D-glucoside pH 8.0, 37°C Bacillus sp. AHU2001
19.2
-
 D-xylose pH 8.0, 37°C Bacillus sp. AHU2001
20.7
-
 L-fucose pH 8.0, 37°C Bacillus sp. AHU2001
28.1
-
 L-sorbose pH 8.0, 37°C Bacillus sp. AHU2001
31.2
-
 D-allose pH 8.0, 37°C Bacillus sp. AHU2001
36.2
-
 kojibiose pH 8.0, 37°C Bacillus sp. AHU2001
40.9
-
 D-mannose pH 8.0, 37°C Bacillus sp. AHU2001
47.8
-
 2-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 SDS-PAGE Bacillus sp. AHU2001
193000
-
 gel filtration Bacillus sp. AHU2001

Organism

Organism UniProt Comment Textmining
Bacillus sp. AHU2001
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-glucitol + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 1,5-anhydro-4-O-alpha-D-glucopyranosyl-D-glucitol + phosphate
-
 ?
2-deoxy-D-glucose + beta-D-glucose 1-phosphate the enzyme synthesizes alpha-(1->3)-glucoside through reverse phosphorolysis with 2-deoxy-D-glucose Bacillus sp. AHU2001 2-deoxy-3-O-alpha-D-glucopyranosyl-D-arabino-hexopyranose + phosphate
-
 ?
3-deoxy-D-glucose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 3-deoxy-4-O-alpha-D-glucopyranosyl-D-ribo-hexopyranose + phosphate
-
 ?
6-deoxy-D-glucose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
 ?
D-allose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-allopyranose + phosphate
-
 ?
D-glucosamine + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 2-amino-2-deoxy-4-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
 ?
D-glucose + beta-D-glucose 1-phosphate
-
 Bacillus sp. AHU2001 maltose + phosphate
-
 ?
D-lyxose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-lyxopyranose + phosphate
-
 ?
D-mannose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-mannopyranose + phosphate
-
 ?
D-xylose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
-
 ?
kojibiose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 1,4-alpha-D-glucopyranosyl-[1,2-alpha-D-glucopyranosyl]-D-glucose + phosphate
-
 ?
L-fucose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-L-fucopyranose + phosphate
-
 ?
L-sorbose + beta-D-glucose 1-phosphate the enzyme synthesizes alpha-(1->3)-glucoside through reverse phosphorolysis with L-sorbose Bacillus sp. AHU2001 1,4-alpha-D-glucopyranosyl-[1,2-beta-D-glucopyranosyl]-D-glucose + phosphate
-
 ?
maltose + phosphate phosphorolytic activity to maltose, but not to other alpha-linked glucobioses and maltotriose. The phosphorolysis of maltose by MalE obeys the sequential bi-bi mechanism Bacillus sp. AHU2001 D-glucose + beta-D-glucose 1-phosphate
-
 ?
methyl alpha-D-glucoside + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 methyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 1 * 90000, SDS-PAGE Bacillus sp. AHU2001

Synonyms

Synonyms Comment Organism
MalE
-
 Bacillus sp. AHU2001

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 phosphorolysis of maltose Bacillus sp. AHU2001

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 50% of maximal activity, 50°C: about 50% of maximal activity Bacillus sp. AHU2001

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 residual activity is higher than 80% of the original activity at temperatures below 40°C after the heat treatment at pH 8.0 for 15 min Bacillus sp. AHU2001

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.436
-
 D-Lyxose pH 8.0, 37°C Bacillus sp. AHU2001
0.568
-
 L-sorbose pH 8.0, 37°C Bacillus sp. AHU2001
0.682
-
 D-allose pH 8.0, 37°C Bacillus sp. AHU2001
0.957
-
 methyl alpha-D-glucoside pH 8.0, 37°C Bacillus sp. AHU2001
1.12
-
 1,5-anhydro-D-glucitol pH 8.0, 37°C Bacillus sp. AHU2001
1.21
-
 L-fucose pH 8.0, 37°C Bacillus sp. AHU2001
8.55
-
 D-mannose pH 8.0, 37°C Bacillus sp. AHU2001
15.8
-
 kojibiose pH 8.0, 37°C Bacillus sp. AHU2001
29.9
-
 D-xylose pH 8.0, 37°C Bacillus sp. AHU2001
30.9
-
 maltose pH 8.0, 37°C Bacillus sp. AHU2001
39.2
-
 2-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
69.1
-
 6-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
107
-
 D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
237
-
 D-glucosamine pH 8.0, 37°C Bacillus sp. AHU2001

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 phosphorolysis of maltose Bacillus sp. AHU2001

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity Bacillus sp. AHU2001

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10.4 the residual activity is higher than 80% of the original activity at a pH range of 4.5-10.4 after the pH treatment at 4°C for 24 h Bacillus sp. AHU2001

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.74
-
 phosphate pH 8.0, 37°C Bacillus sp. AHU2001

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0219
-
 D-allose pH 8.0, 37°C Bacillus sp. AHU2001
0.0289
-
 D-Lyxose pH 8.0, 37°C Bacillus sp. AHU2001
0.0585
-
 L-fucose pH 8.0, 37°C Bacillus sp. AHU2001
0.0687
-
 methyl alpha-D-glucoside pH 8.0, 37°C Bacillus sp. AHU2001
0.0701
-
 L-sorbose pH 8.0, 37°C Bacillus sp. AHU2001
0.0784
-
 1,5-anhydro-D-glucitol pH 8.0, 37°C Bacillus sp. AHU2001
0.209
-
 D-mannose pH 8.0, 37°C Bacillus sp. AHU2001
0.282
-
 3-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
0.436
-
 kojibiose pH 8.0, 37°C Bacillus sp. AHU2001
0.82
-
 2-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
1.57
-
 D-xylose pH 8.0, 37°C Bacillus sp. AHU2001
12.2
-
 6-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
12.8
-
 D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
15.2
-
 D-glucosamine pH 8.0, 37°C Bacillus sp. AHU2001