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Literature summary for 2.4.1.8 extracted from
- Identification of Bacillus selenitireducens MLS10 maltose phosphorylase possessing synthetic ability for branched alpha-D-glucosyl trisaccharides (2012), Carbohydr. Res., 360, 25-30.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Salisediminibacterium selenitireducens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
maltose | pH 6.5, 30°C, phosphorolysis | Salisediminibacterium selenitireducens |  |
| 4.6 | - |
sophorose | pH 6.5, 30°C, reverse phosphorolysis | Salisediminibacterium selenitireducens | |
| 24 | - |
kojibiose | pH 6.5, 30°C, reverse phosphorolysis | Salisediminibacterium selenitireducens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90660, sequence calculation, x * 90000, recombinant His6-tagged Bsel2056, SDS-PAGE | Salisediminibacterium selenitireducens |
| 90660 | - |
x * 90660, sequence calculation, x * 90000, recombinant His6-tagged Bsel2056, SDS-PAGE | Salisediminibacterium selenitireducens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | Salisediminibacterium selenitireducens | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| maltose + phosphate | Salisediminibacterium selenitireducens MLS10 | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| additional information | Salisediminibacterium selenitireducens | the enzyme possesses synthetic ability for alpha-D-glucosyl disaccharides and trisaccharides through the reverse phosphorolysis with beta-D-glucose 1-phosphate as the donor | ? | - |
? | |
| additional information | Salisediminibacterium selenitireducens MLS10 | the enzyme possesses synthetic ability for alpha-D-glucosyl disaccharides and trisaccharides through the reverse phosphorolysis with beta-D-glucose 1-phosphate as the donor | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salisediminibacterium selenitireducens | D6XUS4 | gene bsel2056 | - |
| Salisediminibacterium selenitireducens MLS10 | D6XUS4 | gene bsel2056 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Salisediminibacterium selenitireducens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| maltose + phosphate = D-glucose + beta-D-glucose 1-phosphate | sequential Bi Bi mechanism | Salisediminibacterium selenitireducens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant His-tagged enzyme, 24 h, stable | Salisediminibacterium selenitireducens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetamido-2-deoxy-D-glucose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | ? + phosphate | - |
? | |
| 2-acetamido-2-deoxy-D-glucose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens MLS10 | ? + phosphate | - |
? | |
| 2-amino-2-deoxy-D-glucose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | ? + phosphate | - |
? | |
| 2-amino-2-deoxy-D-glucose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens MLS10 | ? + phosphate | - |
? | |
| 2-deoxy-D-arabino-hexose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | ? + phosphate | - |
? | |
| D-mannose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | ? + phosphate | - |
? | |
| kojibiose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | 1,4-alpha-D-glucopyranosyl-[1,2-alpha-D-glucopyranosyl]-D-glucose + phosphate | - |
? | |
| maltose + phosphate | - |
Salisediminibacterium selenitireducens | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| maltose + phosphate | - |
Salisediminibacterium selenitireducens MLS10 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| additional information | the enzyme possesses synthetic ability for alpha-D-glucosyl disaccharides and trisaccharides through the reverse phosphorolysis with beta-D-glucose 1-phosphate as the donor | Salisediminibacterium selenitireducens | ? | - |
? | |
| additional information | Bsel2056 phosphorolyzes only maltose with inversion of the anomeric configuration releasing beta-D-glucose 1-phosphate and D-glucose. Bsel2056 does not cleave the maltose in the absence of inorganic phosphate. In addition, maltooligosaccharides of degree of polymerization 3-6 are not substrate for Bsel2056. But Bsel2056 shows the flexibility for monosaccharide acceptors with alternative C2 substituent, e.g. 2-amino-2-deoxy-D-glucose, 2-deoxy-D-arabino-hexose, 2-acetamido-2-deoxy-D-glucose, and D-mannose, in the reverse reaction resulting in production of 1,4-alpha-D-glucosyl disaccharides with strict regioselectivity. Bsel2056 possesses a binding space to accommodate the bulky C2 substituent of D-glucose. Substrate specificity and product analysis using NMR, overview | Salisediminibacterium selenitireducens | ? | - |
? | |
| additional information | the enzyme possesses synthetic ability for alpha-D-glucosyl disaccharides and trisaccharides through the reverse phosphorolysis with beta-D-glucose 1-phosphate as the donor | Salisediminibacterium selenitireducens MLS10 | ? | - |
? | |
| additional information | Bsel2056 phosphorolyzes only maltose with inversion of the anomeric configuration releasing beta-D-glucose 1-phosphate and D-glucose. Bsel2056 does not cleave the maltose in the absence of inorganic phosphate. In addition, maltooligosaccharides of degree of polymerization 3-6 are not substrate for Bsel2056. But Bsel2056 shows the flexibility for monosaccharide acceptors with alternative C2 substituent, e.g. 2-amino-2-deoxy-D-glucose, 2-deoxy-D-arabino-hexose, 2-acetamido-2-deoxy-D-glucose, and D-mannose, in the reverse reaction resulting in production of 1,4-alpha-D-glucosyl disaccharides with strict regioselectivity. Bsel2056 possesses a binding space to accommodate the bulky C2 substituent of D-glucose. Substrate specificity and product analysis using NMR, overview | Salisediminibacterium selenitireducens MLS10 | ? | - |
? | |
| sophorose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | 1,4-alpha-D-glucopyranosyl-[1,2-beta-D-glucopyranosyl]-D-glucose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90660, sequence calculation, x * 90000, recombinant His6-tagged Bsel2056, SDS-PAGE | Salisediminibacterium selenitireducens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bsel2056 | - |
Salisediminibacterium selenitireducens |
| inverting maltose phosphorylase | - |
Salisediminibacterium selenitireducens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Salisediminibacterium selenitireducens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant His-tagged enzyme, 15 min, stable | Salisediminibacterium selenitireducens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.6 | - |
sophorose | pH 6.5, 30°C, reverse phosphorolysis | Salisediminibacterium selenitireducens | |
| 12.3 | - |
maltose | pH 6.5, 30°C | Salisediminibacterium selenitireducens | |
| 40 | - |
kojibiose | pH 6.5, 30°C, reverse phosphorolysis | Salisediminibacterium selenitireducens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
reverse reaction | Salisediminibacterium selenitireducens |
| 6.5 | - |
phosphorolysis | Salisediminibacterium selenitireducens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10.5 | purified recombinant His-tagged enzyme, 4°C, 24 h, stable | Salisediminibacterium selenitireducens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the inverting maltose phosphorylase belongs to the glycoside hydrolase family 65, GH65 | Salisediminibacterium selenitireducens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
sophorose | pH 6.5, 30°C, reverse phosphorolysis | Salisediminibacterium selenitireducens | |
| 1.7 | - |
kojibiose | pH 6.5, 30°C, reverse phosphorolysis | Salisediminibacterium selenitireducens | |
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