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Literature summary for 2.4.1.8 extracted from
- The maltodextrin transport system and metabolism in Lactobacillus acidophilus NCFM and production of novel alpha-glucosides through reverse phosphorolysis by maltose phosphorylase (2009), FEBS J., 276, 7353-7365.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene malP, DNA and amino acid sequence analysis, under the control of a transcriptional regulator MalR of the LacIGalR family, sequence comparison. Gene malP belongs to a gene cluster, which is involved in maltodextrin transport and metabolism, overview. Expression of His-tagged MalP in Escherichia coli strain BL21(DE3) | Lactobacillus acidophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
x * 88000, recombinant His-tagged enzyme, SDS-PAGE | Lactobacillus acidophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | Lactobacillus acidophilus | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r |  |
| maltose + phosphate | Lactobacillus acidophilus NCFM | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus acidophilus | Q5FI04 | gene malP | - |
| Lactobacillus acidophilus NCFM | Q5FI04 | gene malP | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged MalP from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus acidophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52 | - |
purified recombinant His-tagged MalP | Lactobacillus acidophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + phosphate | - |
Lactobacillus acidophilus | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| maltose + phosphate | strict specificity for maltose, with no activity using isomaltose, nigerose, kojibiose, and trehalose, but broad specificity of the aglycone binding site | Lactobacillus acidophilus | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| maltose + phosphate | - |
Lactobacillus acidophilus NCFM | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| maltose + phosphate | strict specificity for maltose, with no activity using isomaltose, nigerose, kojibiose, and trehalose, but broad specificity of the aglycone binding site | Lactobacillus acidophilus NCFM | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
| additional information | The enzyme shows reverse phosphorolysis using various carbohydrate acceptor substrates and beta-D-glucose 1-phosphate as the donor, schematic reaction mechanism of reversible phosphorolysis with Glu484 being the general acid catalyst of MalP, overview. For reverse phosphorolysis, the enzyme shows strong preference for monosaccharide acceptors with equatorial 3-OH and 4-OH, such as glucose and mannose. The enzyme also reacts with 2-deoxy glucosamine and 2-deoxy-N-acetylglucosamine, but shows no activity with di- and trisaccharides. Loop 3 of MalP, involved in substrate recognition, blocks the binding of candidate acceptors larger than monosaccharides, molecular modelling, overview. MalP efficiently catalyses the synthesis of several alpha-glucosidic disaccharides, i.e. of alpha-Glc-phosphate-(1,4)-Glc-phosphate, alpha-Glc-phosphate-(1,4)-GlcN-phosphate, alpha-Glc-phosphate-(1,4)-GlcNAc-phosphate, alpha-Glc-phosphate-(1,4)-Man-phosphate, alpha-Glc-phosphate-(1,4)-Xyl-phosphate and alpha-Glc-phosphate-(1,4)-l-Fuc-phopshate, determined by NMR and mass spectrometry, overview. Structural basis for MalP specificity, overview | Lactobacillus acidophilus | ? | - |
? | |
| additional information | The enzyme shows reverse phosphorolysis using various carbohydrate acceptor substrates and beta-D-glucose 1-phosphate as the donor, schematic reaction mechanism of reversible phosphorolysis with Glu484 being the general acid catalyst of MalP, overview. For reverse phosphorolysis, the enzyme shows strong preference for monosaccharide acceptors with equatorial 3-OH and 4-OH, such as glucose and mannose. The enzyme also reacts with 2-deoxy glucosamine and 2-deoxy-N-acetylglucosamine, but shows no activity with di- and trisaccharides. Loop 3 of MalP, involved in substrate recognition, blocks the binding of candidate acceptors larger than monosaccharides, molecular modelling, overview. MalP efficiently catalyses the synthesis of several alpha-glucosidic disaccharides, i.e. of alpha-Glc-phosphate-(1,4)-Glc-phosphate, alpha-Glc-phosphate-(1,4)-GlcN-phosphate, alpha-Glc-phosphate-(1,4)-GlcNAc-phosphate, alpha-Glc-phosphate-(1,4)-Man-phosphate, alpha-Glc-phosphate-(1,4)-Xyl-phosphate and alpha-Glc-phosphate-(1,4)-l-Fuc-phopshate, determined by NMR and mass spectrometry, overview. Structural basis for MalP specificity, overview | Lactobacillus acidophilus NCFM | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, recombinant His-tagged enzyme, SDS-PAGE | Lactobacillus acidophilus |
| More | homology modelling | Lactobacillus acidophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GH65 maltose phosphorylase | - |
Lactobacillus acidophilus |
| MalP | - |
Lactobacillus acidophilus |
| More | MalP belongs to the glycoside hydrolase family 65, GH65 | Lactobacillus acidophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant His-tagged MalP | Lactobacillus acidophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 75 | MalP activity range with substrate maltose | Lactobacillus acidophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 90 | purified recombinant enzyme, thermostability profile, pH 6.2 | Lactobacillus acidophilus |
| 45 | - |
15 min, purified recombinant enzyme, stable up to | Lactobacillus acidophilus |
| 57 | - |
15 min, purified recombinant enzyme, 50% remaining activity | Lactobacillus acidophilus |
| 65 | - |
15 min, purified recombinant enzyme, 10% remaining activity | Lactobacillus acidophilus |
| 90 | - |
15 min, inactivation | Lactobacillus acidophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
recombinant His-tagged MalP | Lactobacillus acidophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8.5 | MalP activity range with substrate maltose | Lactobacillus acidophilus |
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