Literature summary for 2.4.1.79 extracted from

Taniguchi, N.; Makita, A.
Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase, a synthase of human blood group P antigen, from canine spleen (1984), J. Biol. Chem., 259, 5637-5642.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	10 mM, stimulation to 120% of the original activity	Canis lupus familiaris

General Stability

General Stability	Organism
diluted enzyme solutions, below 0.1 mg protein/ml, unstable	Canis lupus familiaris
Stable to repeated freeze-thawing	Canis lupus familiaris

Inhibitors

Inhibitors	Comment	Organism	Structure
4-hydroxymercuribenzoate	0.5 mM, complete inhibition	Canis lupus familiaris
EDTA	-	Canis lupus familiaris
iodoacetamide	1 mM, 65% inhibition	Canis lupus familiaris
iodoacetic acid	1 mM, 91% inhibition	Canis lupus familiaris
NEM	1 mM, 67% inhibition	Canis lupus familiaris
UDP	competitive to UDP-N-acetylgalactosamine, non-competitive to globotriaosylceramide	Canis lupus familiaris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0025	-	D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide	-	Canis lupus familiaris
0.014	-	UDP-N-acetyl-alpha-D-glucosamine	-	Canis lupus familiaris

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Canis lupus familiaris	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	43% of the activation with Mn2+	Canis lupus familiaris
Fe2+	19% of the activation with Mn2+	Canis lupus familiaris
Li+	11% of the activation with Mn2+	Canis lupus familiaris
Mn2+	required	Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	x * 120000, SDS-PAGE without prior reduction	Canis lupus familiaris

Organism

Organism	UniProt	Comment	Textmining
Canis lupus familiaris	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Canis lupus familiaris

Source Tissue

Source Tissue	Comment	Organism	Textmining
spleen	-	Canis lupus familiaris	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000565	-	-	Canis lupus familiaris

Storage Stability

Storage Stability	Organism
-80°C, above 0.1 mg protein/ml in 25 mM sodium cacodylate buffer, pH 6.9, 1% Triton X-100 and 20% glycerol, stable for at least 3 months	Canis lupus familiaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme catalyzes the conversion of human blood group Pk antigen into P antigen	Canis lupus familiaris	?	-	?
UDP-N-acetyl-alpha-D-glucosamine + globotriaosylceramide	-	Canis lupus familiaris	?	-	?
UDP-N-acetyl-D-galactosamine + D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide	UDP-N-acetyl-D-galactosamine is the sole sugar donor	Canis lupus familiaris	UDP + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide	-	?

Subunits

Subunits	Comment	Organism
?	x * 120000, SDS-PAGE without prior reduction	Canis lupus familiaris

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.9	-	-	Canis lupus familiaris

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Release 2023.1 (January 2023)