Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | Staphylococcus aureus | |
Co2+ | slight activation | Staphylococcus aureus | |
Mg2+ | 8 mM, required for activity | Staphylococcus aureus | |
Mn2+ | slight activation | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Staphylococcus aureus | |
Fe2+ | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
poly(ribitol phosphate) | - |
Staphylococcus aureus | |
0.7 | - |
UDP-N-acetylglucosamine | - |
Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + poly(ribitol phosphate) | Staphylococcus aureus | D-alanyl-N-acetyl-alpha-galactosamine poly(ribitol phosphate) is a less efficient acceptor, poor donor substrate is UDPglucose, no activity with teichoic acid, D-ribitol 5-phosphate or partial alkali hydrolysates of teichoic acid or N-acetyl-galactosamine polyribitol phosphate, UDP-N-acetyl-galactosamine, N-acetyl-galactosamine and N-acetyl-galactosamine phosphate | UDP + N-acetyl-D-glucosaminyl-poly(ribitol phosphate) | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
strains H or Duncan: primarily N-acetyl-beta-glucosamine transfer, strain 3528: N-acetyl-alpha-glucosamine transfer, strain Copenhagen: both transferases | - |
Storage Stability | Organism |
---|---|
frozen, N-acetyl-alpha-D-glucosamine transferase, not N-acetyl-beta-D-glucosamine transferase, a few months | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + poly(ribitol phosphate) | 10% of activity with UDP-N-acetyl-D-glucosamine | Staphylococcus aureus | UDP + glucosyl-poly(ribitol phosphate) | - |
? | |
UDP-N-acetyl-D-glucosamine + poly(ribitol phosphate) | D-alanyl-N-acetyl-alpha-galactosamine poly(ribitol phosphate) is a less efficient acceptor, poor donor substrate is UDPglucose, no activity with teichoic acid, D-ribitol 5-phosphate or partial alkali hydrolysates of teichoic acid or N-acetyl-galactosamine polyribitol phosphate, UDP-N-acetyl-galactosamine, N-acetyl-galactosamine and N-acetyl-galactosamine phosphate | Staphylococcus aureus | UDP + N-acetyl-D-glucosaminyl-poly(ribitol phosphate) | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Staphylococcus aureus |