Literature summary for 2.4.1.7 extracted from

Wang, M.; Wu, J.; Wu, D.
Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme (2018), Microb. Cell Fact., 17, 23 .

Search for more literature for 2.4.1.7

Application

Application	Comment	Organism
synthesis	effective kojibiose synthesis i as performed using the crude enzyme solution from the supernatant of fermentation broth of Bacillus subtilis expressing the enzyme from Bifidobacterium adolescentis, providing a basis for potential industrial-scale preparation of kojibiose. Kojibiose is prebiotic element, a low-calorie sweetener and exhibits antitoxic activity, thereby promoting the emergence of new drugs	Bifidobacterium adolescentis

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression in a food-grade Bacillus subtilis strain. Efficiently secreted into the extracellular medium in the absence of a signal peptide. After culturing the recombinant strain in a 3-l bioreactor, crude enzyme yield and activity reach 7.5 g/l and 5.3 U/ml, respectively	Bifidobacterium adolescentis

Organism

Organism	UniProt	Comment	Textmining
Bifidobacterium adolescentis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + D-glucose	kojibiose i.e. 2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranose	Bifidobacterium adolescentis	D-fructose + 2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranose	-	?
sucrose + phosphate	-	Bifidobacterium adolescentis	D-fructose + alpha-D-glucose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SPase	-	Bifidobacterium adolescentis

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Release 2023.1 (January 2023)