Protein Variants | Comment | Organism |
---|---|---|
D49A | site-directed mutagenesis, the mutant enzyme shows 10000000fold reduced enzyme glycosylation and 500fold reduced enzyme deglycosylation compared to the wild-type enzyme. The mutant also shows a loss in selectivity for phosphate against water and substrate inhibition by phosphate | Leuconostoc mesenteroides |
D49A/R395L | site-directed mutagenesis, inactive mutant | Leuconostoc mesenteroides |
R395L | site-directed mutagenesis, the mutant enzyme shows 100000fold reduced enzyme glycosylation and 500fold reduced enzyme deglycosylation compared to the wild-type enzyme. The mutant also shows a loss in selectivity for phosphate against water and substrate inhibition by phosphate | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | the transferred glucosyl moiety of sucrose is accomodated at the catalytic subsite of the phosphorylase through a network of charged hydrogen bonds, conserved residues Asp49 and Arg395 are pointing towards the equatorial hydroxyl at C4 which is essential for catalytic efficiency, overview | Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | sucrose phosphorylase is a bacterial alpha-transglucosidase that catalyses glucosyl transfer from sucrose to phosphate, releasing D-fructose and alpha-glucose 1-phosphate as product of the first enzyme glycosylation step and second enzyme deglycosylation step of the enzymatic reaction, respectively | Leuconostoc mesenteroides |