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Literature summary for 2.4.1.7 extracted from
- Cloning and expression of the sucrose phosphorylase gene from Leuconostoc mesenteroides in Escherichia coli (2008), Biotechnol. Lett., 30, 749-754.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the His4-tagged enzyme in Escherichia coli strain DH5alpha | Leuconostoc mesenteroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
sucrose | pH 6.7, 37°C, His4-tagged enzyme | Leuconostoc mesenteroides |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59300 | - |
x * 59300, recombinant His4-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
| sucrose + phosphate | Leuconostoc mesenteroides NRRL B-742 | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
- |
- |
| Leuconostoc mesenteroides NRRL B-742 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His4-tagged enzyme from Escherichia coli | Leuconostoc mesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 285 | - |
purified recombinant His4-tagged enzyme | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + CMP | - |
Leuconostoc mesenteroides | D-fructose + CMP 1-glucoside | - |
? | |
| sucrose + CMP | - |
Leuconostoc mesenteroides NRRL B-742 | D-fructose + CMP 1-glucoside | - |
? | |
| sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
| sucrose + phosphate | - |
Leuconostoc mesenteroides NRRL B-742 | D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59300, recombinant His4-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 742SPase | - |
Leuconostoc mesenteroides |
| More | the enzyme belongs to family 13 of the glycosyl hydrolases | Leuconostoc mesenteroides |
| SPase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
recombinant enzyme | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
recombinant His4-tagged enzyme, stable up to | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
recombinant enzyme | Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | recombinant His4-tagged enzyme, stable | Leuconostoc mesenteroides |
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