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Literature summary for 2.4.1.7 extracted from

  • Fujii, K.; Iiboshi, M.; Yanase, M.; Takaha, T.; Kuriki, T.
    Enhancing the thermal stability of sucrose phosphorylase from Streptococcus mutans by random mutagenesis (2006), J. Appl. Glycosci., 53, 91-97.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
D249G mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
K140M mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
N155S mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
Q144R mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
S62P mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
T47S mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G mutant enzyme retains more than 60% of initial activity at 60°C for 20 min Streptococcus mutans
V128L mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans
Y77H mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
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Purification (Commentary)

Purification (Comment) Organism
purification of thermostable mutant T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G by heating at 65°C for 20 min with 20% sucrose. The purified enzyme can be directly employed for the production of amylose with further purification Streptococcus mutans