Protein Variants | Comment | Organism |
---|---|---|
D249G | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
K140M | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
N155S | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
Q144R | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
S62P | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
T47S | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G | mutant enzyme retains more than 60% of initial activity at 60°C for 20 min | Streptococcus mutans |
V128L | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
Y77H | mutation contributes to the enhancement of thermal stability, mutant enzyme retains activity after heat treatment at 55°C for 20 min | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of thermostable mutant T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G by heating at 65°C for 20 min with 20% sucrose. The purified enzyme can be directly employed for the production of amylose with further purification | Streptococcus mutans |