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Literature summary for 2.4.1.7 extracted from
- Cloning and expression of sucrose phosphorylase gene from Bifidobacterium longum in E. coli and characterization of the recombinant enzyme (2003), Biotechnol. Lett., 25, 1211-1217.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from genetic library, complementation of growth deficient Escherichia coli strain JM109, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Bifidobacterium longum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, recombinant enzyme, SDS-PAGE | Bifidobacterium longum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | - |
sttrain SJ32, gene splP | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme partially by anion exchange chromatography | Bifidobacterium longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | hydrolytic activity | Bifidobacterium longum | 4-nitrophenol + alpha-D-glucose | - |
? |  |
| 4-nitrophenyl-alpha-D-galactopyranoside + H2O | hydrolytic activity | Bifidobacterium longum | 4-nitrophenol + alpha-D-galactose | - |
? | |
| additional information | the recombinant enzyme shows no activity with melibiose, melezitose, and raffinose, and exhibits transglucosylation activity in addition to hydrolytic activity | Bifidobacterium longum | ? | - |
? | |
| sucrose + phosphate | recombinant enzyme | Bifidobacterium longum | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, recombinant enzyme, SDS-PAGE | Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at, activity with oligosaccharides | Bifidobacterium longum |
| 37 | - |
assay at, hydrolytic activity with 4-nitrophenyl-alpha-D-galactopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside as substrates | Bifidobacterium longum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
assay at | Bifidobacterium longum |
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