Cloned (Comment) | Organism |
---|---|
cloning from genetic library, complementation of growth deficient Escherichia coli strain JM109, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Bifidobacterium longum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, recombinant enzyme, SDS-PAGE | Bifidobacterium longum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum | - |
sttrain SJ32, gene splP | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially by anion exchange chromatography | Bifidobacterium longum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | hydrolytic activity | Bifidobacterium longum | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-nitrophenyl-alpha-D-galactopyranoside + H2O | hydrolytic activity | Bifidobacterium longum | 4-nitrophenol + alpha-D-galactose | - |
? | |
additional information | the recombinant enzyme shows no activity with melibiose, melezitose, and raffinose, and exhibits transglucosylation activity in addition to hydrolytic activity | Bifidobacterium longum | ? | - |
? | |
sucrose + phosphate | recombinant enzyme | Bifidobacterium longum | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, recombinant enzyme, SDS-PAGE | Bifidobacterium longum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, activity with oligosaccharides | Bifidobacterium longum |
37 | - |
assay at, hydrolytic activity with 4-nitrophenyl-alpha-D-galactopyranoside and 4-nitrophenyl-alpha-D-glucopyranoside as substrates | Bifidobacterium longum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
assay at | Bifidobacterium longum |