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Literature summary for 2.4.1.7 extracted from
- Physico-chemical and transglucosylation properties of recombinant sucrose phosphorylase from Bifidobacterium adolescentis DSM20083 (2004), Appl. Microbiol. Biotechnol., 65, 219-227.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sucP, cloning from genomic library, DNA and amino acid sequence determination and analysis, determination of promotor region, expression in Escherichia coli | Bifidobacterium adolescentis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bifidobacterium adolescentis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56189 | - |
2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation | Bifidobacterium adolescentis |
| 58000 | - |
2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation | Bifidobacterium adolescentis |
| 129000 | - |
gel filtration | Bifidobacterium adolescentis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + phosphate | Bifidobacterium adolescentis | - |
beta-D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium adolescentis | Q84HQ2 | DSM20083, gene sucP | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Bifidobacterium adolescentis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 109 | - |
purified recombinant enzyme, substrate sucrose | Bifidobacterium adolescentis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | alpha-D-Glc(1-1)-beta-D-Ara + phosphate | - |
? | |
| D-arabitol + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-fructose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-fucose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-galactose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-glucose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-sorbitol + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-xylitol + alpha-D-glucose 1-phosphate | high activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| D-xylose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| L-arabinose + alpha-D-glucose 1-phosphate | high activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| L-arabitol + alpha-D-glucose 1-phosphate | high activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| L-fucose + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| L-sorbose + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| L-xylose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
| additional information | substrate specificity, di- and trisaccharides, including sucrose, are no acceptor substrate, overview | Bifidobacterium adolescentis | ? | - |
? | |
| sucrose + phosphate | - |
Bifidobacterium adolescentis | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
| sucrose + phosphate | enzyme prefers the forward reaction | Bifidobacterium adolescentis | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation | Bifidobacterium adolescentis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the glycoside hydrolase family 13 | Bifidobacterium adolescentis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
- |
Bifidobacterium adolescentis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Bifidobacterium adolescentis |
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