Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and JM109 (DE3) cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | less than 20% residual activity at 2 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
GDP-beta-L-fucose | at pH 7.6 and 30°C | Escherichia coli | |
5.3 | - |
D-lactose | at pH 7.6 and 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows no strict requirement for divalent metal ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, calculated from amino acid sequence | Escherichia coli |
70000 | - |
x * 70000, recombinant, His- and pro-protein-tagged enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | Escherichia coli | - |
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? | |
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | Escherichia coli O126 | - |
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | A6M9C2 | - |
- |
Escherichia coli O126 | A6M9C2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl buffer pH 7.6, 2 months, 30% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
Escherichia coli | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? | |
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
Escherichia coli O126 | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide | - |
? | |
GDP-beta-L-fucose + D-galactose | 70% activity compared to D-lactose | Escherichia coli | ? | - |
? | |
GDP-beta-L-fucose + D-galactose | 70% activity compared to D-lactose | Escherichia coli O126 | ? | - |
? | |
GDP-beta-L-fucose + D-lactose | 100% activity | Escherichia coli | GDP + 2'-fucosyl-D-lactose | - |
? | |
GDP-beta-L-fucose + D-lactose | 100% activity | Escherichia coli O126 | GDP + 2'-fucosyl-D-lactose | - |
? | |
GDP-beta-L-fucose + D-lactulose | 125% activity compared to D-lactose | Escherichia coli | GDP + 2'-fucosyl-D-lactulose | - |
? | |
GDP-beta-L-fucose + D-lactulose | 125% activity compared to D-lactose | Escherichia coli O126 | GDP + 2'-fucosyl-D-lactulose | - |
? | |
GDP-beta-L-fucose + Gal(beta1-3)GlcNAc | 28% activity compared to D-lactose | Escherichia coli | ? | - |
? | |
GDP-beta-L-fucose + Gal(beta1-4)GlcNAc | 85% activity compared to D-lactose | Escherichia coli | ? | - |
? | |
GDP-beta-L-fucose + Galbeta-4-nitrophenyl | 28% activity compared to D-lactose | Escherichia coli | ? | - |
? | |
additional information | no activity with D-galactosamine, N-acetyl-D-galactosamine, Gal(beta1-4)Glcbeta1-Bn, Gal(beta1-4)Glcbeta1-octyl, Gal(beta1-4)[Fuc(alpha1-3)]Glc, Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)GlcNAcbeta-linker-tBoc, and Gal(alpha1-6)Glc(alpha1-2)Fru | Escherichia coli | ? | - |
? | |
additional information | no activity with D-galactosamine, N-acetyl-D-galactosamine, Gal(beta1-4)Glcbeta1-Bn, Gal(beta1-4)Glcbeta1-octyl, Gal(beta1-4)[Fuc(alpha1-3)]Glc, Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)GlcNAcbeta-linker-tBoc, and Gal(alpha1-6)Glc(alpha1-2)Fru | Escherichia coli O126 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, calculated from amino acid sequence | Escherichia coli |
? | x * 70000, recombinant, His- and pro-protein-tagged enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alpha1,2-fucosyltransferase | - |
Escherichia coli |
alpha1,2-FucT | - |
Escherichia coli |
WbgL | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
at 27°C in 50 mM Tris-HCl buffer pH 7.6, the enzyme shows a half -life of 57 h | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
GDP-beta-L-fucose | at pH 7.6 and 30°C | Escherichia coli | |
0.067 | - |
D-lactose | at pH 7.6 and 30°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | calculated from amino acid sequence | - |
6.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
D-lactose | at pH 7.6 and 30°C | Escherichia coli | |
0.245 | - |
GDP-beta-L-fucose | at pH 7.6 and 30°C | Escherichia coli |