Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BYC5 | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-fucose + Gal-N-acetylglucosaminyl-Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine | 100% yield | Homo sapiens | GDP + L-fucose alpha-1,6 bound to Gal-N-acetylglucosaminyl-Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine | - |
? | |
GDP-beta-fucose + Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine | 30% yield | Homo sapiens | GDP + L-fucose alpha-1,6 bound to Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine | - |
? | |
GDP-beta-L-fucose + Gal-N-acetylglucosaminyl-Man5-GlcNAc2-HIV-1 V3 glycopeptide | 100% yield | Homo sapiens | GDP + L-fucose alpha-1,6-bound to Gal-N-acetylglucosaminyl-Man5-GlcNAc2-HIV-1 V3 glycoprotein | - |
? | |
GDP-beta-L-fucose + Man5-GlcNAc2-erythropoietin | FUT8 is able to core-fucosylate Man5GlcNAc2 glycan in vitro in the context of reythropoietin, lacking an unmodified GlcNAc moiety at the alpha1,3-arm, 90% yield | Homo sapiens | GDP + L-fucose alpha-1,6-bound to Man5-GlcNAc2-erythropoietin | - |
? | |
GDP-beta-L-fucose + Man5-GlcNAc2-HIV-1 V3 glycopeptide | 50% yield | Homo sapiens | GDP + L-fucose alpha-1,6-bound to Man5-GlcNAc2-HIV-1 V3 glycoprotein | - |
? | |
additional information | free N-glycans lacking an unmasked alpha-1,3-arm GlcNAc moiety are not FUT8 substrates. Man5GlcNAc2 glycan can be efficiently core-fucosylated by FUT8 in an appropriate protein/peptide context, such as with the erythropoietin protein, a V3 polypeptide derived from HIV-1 gp120, or a simple 9-fluorenylmethyl chloroformate-protected Asn moiety. When placed in the V3 polypeptide context, a mature bi-antennary complex-type N-glycan also can be core-fucosylated by FUT8, albeit at much lower efficiency than the Man5GlcNAc2 peptide | Homo sapiens | ? | - |
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