Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.68 extracted from

  • Yang, Q.; Zhang, R.; Cai, H.; Wang, L.X.
    Revisiting the substrate specificity of mammalian alpha1,6-fucosyltransferase reveals that it catalyzes core fucosylation of N-glycans lacking alpha1,3-arm GlcNAc (2017), J. Biol. Chem., 292, 14796-14803 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.68

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BYC5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-fucose + Gal-N-acetylglucosaminyl-Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine 100% yield Homo sapiens GDP + L-fucose alpha-1,6 bound to Gal-N-acetylglucosaminyl-Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine
-
 ?
GDP-beta-fucose + Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine 30% yield Homo sapiens GDP + L-fucose alpha-1,6 bound to Man5-GlcNAc2-(N-(9-fluorenyl)methoxycarbonyl)asparagine
-
 ?
GDP-beta-L-fucose + Gal-N-acetylglucosaminyl-Man5-GlcNAc2-HIV-1 V3 glycopeptide 100% yield Homo sapiens GDP + L-fucose alpha-1,6-bound to Gal-N-acetylglucosaminyl-Man5-GlcNAc2-HIV-1 V3 glycoprotein
-
 ?
GDP-beta-L-fucose + Man5-GlcNAc2-erythropoietin FUT8 is able to core-fucosylate Man5GlcNAc2 glycan in vitro in the context of reythropoietin, lacking an unmodified GlcNAc moiety at the alpha1,3-arm, 90% yield Homo sapiens GDP + L-fucose alpha-1,6-bound to Man5-GlcNAc2-erythropoietin
-
 ?
GDP-beta-L-fucose + Man5-GlcNAc2-HIV-1 V3 glycopeptide 50% yield Homo sapiens GDP + L-fucose alpha-1,6-bound to Man5-GlcNAc2-HIV-1 V3 glycoprotein
-
 ?
additional information free N-glycans lacking an unmasked alpha-1,3-arm GlcNAc moiety are not FUT8 substrates. Man5GlcNAc2 glycan can be efficiently core-fucosylated by FUT8 in an appropriate protein/peptide context, such as with the erythropoietin protein, a V3 polypeptide derived from HIV-1 gp120, or a simple 9-fluorenylmethyl chloroformate-protected Asn moiety. When placed in the V3 polypeptide context, a mature bi-antennary complex-type N-glycan also can be core-fucosylated by FUT8, albeit at much lower efficiency than the Man5GlcNAc2 peptide Homo sapiens ?
-
-