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Literature summary for 2.4.1.68 extracted from
- Identification of a GDP-L-fucose:polypeptide fucosyltransferase and enzymic addition of O-linked fucose to EGF domains (1996), Glycobiology, 6, 837-842.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | enzyme has a cytosolic part and a stem region which is very suceptible to proteolysis | Cricetulus griseus | 16020 | - |
| membrane | enzyme has a cytosolic part and a stem region which is very suceptible to proteolysis | Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Cricetulus griseus |  |
| Mn2+ | activation, 10fold at 50 mM | Cricetulus griseus | |
| Mn2+ | activation, 10fold at 50 mM | Rattus norvegicus | |
| additional information | no effect of EDTA | Cricetulus griseus | |
| Ni2+ | activation | Cricetulus griseus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially from CHO cell culture | Cricetulus griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| CHO cell | - |
Cricetulus griseus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay method development | Cricetulus griseus |
| additional information | - |
assay method development | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, no loss of activity, at least 1 month | Cricetulus griseus |
| -20°C, no loss of activity, at least 1 month | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-L-fucose + factor VII EGF-1 domain | recombinant human acceptor substrate, with and without His-tag | Cricetulus griseus | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain | fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue | ? | |
| GDP-L-fucose + factor VII EGF-1 domain | recombinant human acceptor substrate, with and without His-tag | Rattus norvegicus | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain | fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue | ? | |
| additional information | synthetic peptides of 5 proteins which contain O-linked fucose do not serve as acceptor substrates, overview | Cricetulus griseus | ? | - |
? | |
| additional information | synthetic peptides of 5 proteins which contain O-linked fucose do not serve as acceptor substrates, overview | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetulus griseus |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Cricetulus griseus |
| 7 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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