Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.66 extracted from

  • Myllylä, R.; Anttinen, H.; Risteli, L.; Kivirikko, K.I.
    Isolation of collagen glucosyltransferase as a homogeneous protein from chick embryos (1977), Biochim. Biophys. Acta, 480, 113-121.
    View publication on PubMed
Search for more literature for 2.4.1.66

General Stability

General Stability Organism
concentrated enzyme, above 0.2 mg/ml, loses most of the activity Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 white Leghorn chicken
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
14600fold Gallus gallus
affinity chromatography Gallus gallus
solubilized with 0.5 M NaCl, 2 M urea increases solubilization rate Gallus gallus
solubilized with Nonidet P-40 Gallus gallus
solubilized with Triton X-100 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.43
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, enzyme concentration 0.1 mg/ml, several months, 33% loss of activity Gallus gallus
4°C, enzyme concentration 0.1 mg/ml, 1 week, 33% loss of activity Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine Gallus gallus ?
-
 ?
additional information not: ADPglucose Gallus gallus ?
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 72000-78000, value depends on the gel composition, SDS-PAGE Gallus gallus