Any feedback?
Literature summary for 2.4.1.66 extracted from
- Metal activation of galactosylhydroxylysyl glucosyltransferase, an intracellular enzyme of collagen biosynthesis (1979), Eur. J. Biochem., 101, 261-269.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 50% activation of pure enzyme | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5-10 mM: 10-15% inhibition of Mn2+-activated enzyme | Gallus gallus |  |
| Cd2+ | inhibits Mn2+-activated enzyme | Gallus gallus | |
| Co2+ | inhibits at high concentrations, inhibits Mn2+-activated enzyme | Gallus gallus | |
| Cu2+ | inhibits Mn2+-activated enzyme | Gallus gallus | |
| EDTA | - |
Gallus gallus | |
| Fe2+ | inhibits at high concentrations | Gallus gallus | |
| Fe3+ | inhibits Mn2+-activated enzyme, 0.03 mM: 27% inhibition of activity with 0.01 mM Mn2+ and 0.035 mM UDP-glucose | Gallus gallus | |
| Mn2+-UDP-complex | competitive inhibition to UDP-glucose, non-competitive to collagen | Gallus gallus | |
| additional information | not inhibited by Mg2+ | Gallus gallus | |
| Ni2+ | inhibits Mn2+-activated enzyme | Gallus gallus | |
| UDP | competitive to UDP-glucose; non-competitive to collagen; product inhibition | Gallus gallus | |
| Zn2+ | kinetics | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | 0.007 | UDP-glucose | at high Mn2+-concentration, 2 Mn2+ bound | Gallus gallus | |
| 0.025 | - |
UDP-glucose | at low Mn2+-concentration, 1 Mn2+ bound | Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | located within the cisternae of the endoplasmic reticulum | Gallus gallus | 5783 | - |
| intracellular | - |
Gallus gallus | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates at low but inhibits at high concentrations, optimal concentration: 0.3 mM, 80% as effective as 0.01 mM Mn2+ | Gallus gallus | |
| Fe2+ | optimal concentration: 0.03 mM, activates at low but inhibits at high concentrations | Gallus gallus | |
| Mn2+ | requirement | Gallus gallus | |
| Mn2+ | activates, 2 Mn2+-ions bound per enzyme molecule, mechanism | Gallus gallus | |
| Mn2+ | 0.01 mM | Gallus gallus | |
| additional information | not activated by Ca2+ | Gallus gallus | |
| additional information | not activated by Ni2+ | Gallus gallus | |
| additional information | not activated by Mg2+ | Gallus gallus | |
| additional information | not activated by Cd2+ | Gallus gallus | |
| additional information | not activated by Zn2+, Cu2+ | Gallus gallus | |
| additional information | not activated by Fe3+ | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Gallus gallus | intracellular enzyme of collagen biosynthesis | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-glucose + [procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine = UDP + [procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine | ordered mechanism | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | ordered binding of Mn2+, UDP-glucose and collagen at low Mn2+ concentration and of two Mn2+, UDP-glucose and collagen at high Mn2+ concentration | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | heat-denatured citrate-soluble rat skin collagen | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | product is glucosylgalactosylhydroxylysine-collagen | ? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | intracellular enzyme of collagen biosynthesis | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
UDP | - |
Gallus gallus | |
| 0.004 | - |
Mn2+-UDP-complex | - |
Gallus gallus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
