KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.167 | - |
Collagen | denatured citrate-soluble calf skin collagen | Bos taurus | |
0.28 | - |
Collagen | acetic acid-soluble collagen from alkali-treated calf arterial tissue | Bos taurus | |
0.33 | - |
Collagen | beta12-component of calf skin collagen | Bos taurus | |
0.63 | - |
Collagen | alpha-chain of calf skin collagen | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50-60% as effective as Mn2+ | Bos taurus | |
Mg2+ | 50-60% as effective as Mn2+ | Bos taurus | |
Mn2+ | requirement | Bos taurus | |
Mn2+ | 10 mM | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Bos taurus | glycosylation in the course of procollagen and collagen biosynthesis, carbohydrate attachment is an essential step in the protocollagen biosynthesis, postribosomal modification | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
50fold | Bos taurus |
partial | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta thoracica | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, 0.1 M Tris-buffer, pH 7.2, 0.2 M NaCl, 1 week, 10% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + collagen | - |
Bos taurus | ? | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | requires UDP-glucose as glucosyl donor and acceptor protein with terminal galactose residues | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acetic acid-soluble collagen from alkali-treated calf arterial tissue | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | denatured citrate-soluble calf skin collagen is the best substrate, its alpha1-chain and beta12-component are also acceptors, but with lower affinity | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | glycosylation in the course of procollagen and collagen biosynthesis, carbohydrate attachment is an essential step in the protocollagen biosynthesis, postribosomal modification | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + asialo alpha 1-glycoprotein | sialic acid-free alpha1-acid glycoprotein of human serum, 1/50 of affinity of citrate-soluble calf skin collagen | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
denaturation at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.2 and 8.1 | Bos taurus |
8.3 | - |
2 pH-optima: 8.3 and 9.9 | Bos taurus |
9.9 | - |
2 pH-optima: 8.3 and 9.9 | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11.5 | about half-maximal activity at pH 4.0 and 11.5 | Bos taurus |