Cloned (Comment) | Organism |
---|---|
cDNA sequence of GGT/LH3 | Homo sapiens |
LH cDNA with GGT activity is expressed in Escherichia coli XL1-Blue, cDNA sequence of GGT/LH | Caenorhabditis elegans |
LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A361G | mutant with reduced GGT activity | Caenorhabditis elegans |
A380V | mutant with reduced GGT activity | Caenorhabditis elegans |
A453I | mutant with a remarkably reduced GGT activity | Caenorhabditis elegans |
A464I | no effect on GGT activity | Homo sapiens |
C132I | mutant with dramatically reduced GGT activity | Caenorhabditis elegans |
C144I | mutant with dramatically reduced GGT activity | Homo sapiens |
D331V | little effect on GGT activity | Caenorhabditis elegans |
D392A | little effect on GGT activity | Homo sapiens |
E371T | mutant with reduced GGT activity | Caenorhabditis elegans |
E547K | mutant with increased GGT activity | Caenorhabditis elegans |
F131L | mutant with markedly reduced GGT activity | Caenorhabditis elegans |
F143L | mutant with reduced GGT activity | Homo sapiens |
F372Y | little effect on GGT activity | Caenorhabditis elegans |
H165S | mutant with reduced GGT activity | Caenorhabditis elegans |
K482A | mutant with increased GGT activity | Caenorhabditis elegans |
K674V | no effect on GGT activity | Caenorhabditis elegans |
L124V | mutant with 40% inhibited GGT activity | Caenorhabditis elegans |
L125V | mutant with 70% inhibited GGT activity | Caenorhabditis elegans |
L136V | mutant with increased GGT activity | Homo sapiens |
L137V | mutant with increased GGT activity | Homo sapiens |
L192K | mutant with reduced GGT activity | Caenorhabditis elegans |
L196I | mutant with markedly reduced GGT activity | Caenorhabditis elegans |
L208I | mutant with markedly reduced GGT activity | Homo sapiens |
L42K | mutant with increased GGT activity | Caenorhabditis elegans |
L491M | mutant with little increased GGT activity | Caenorhabditis elegans |
L562I | mutant with increased GGT activity | Caenorhabditis elegans |
L591I | mutant with increased GGT activity | Caenorhabditis elegans |
M406L | mutant with reduced GGT activity | Caenorhabditis elegans |
M642L | mutant with increased GGT activity | Caenorhabditis elegans |
M650L | no effect on GGT activity | Homo sapiens |
M724A | little effect on GGT activity | Caenorhabditis elegans |
additional information | mutagenesis of aspartates of the DXD-like motif in LH3 eliminates GGT activity | Homo sapiens |
P591N | no effect on GGT activity | Caenorhabditis elegans |
Q613E | mutant with little increased GGT activity | Caenorhabditis elegans |
S204C | mutant with reduced GGT activity | Caenorhabditis elegans |
V622L | no effect on GGT activity | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
soluble | - |
Caenorhabditis elegans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Caenorhabditis elegans | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the amino acids important for the GGT activity are located in the N-terminal part of LH3/GGT protein separate from the C-terminal LH active site | Homo sapiens | ? | - |
? | |
additional information | lysyl hydroxylase and collagen glucosyltransferase activities are associated with the same gene product, the amino acids important for the GGT activity are located in the N-terminal part of LH/GGT protein separate from the C-terminal LH active site, C-132 and L-196 are important for the catalytic activity of GGT | Caenorhabditis elegans | ? | - |
? | |
additional information | multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase and collagen glucosyltransferase activities | Homo sapiens | ? | - |
? | |
additional information | C-144 and L-208 are important for the catalytic activity of GGT, DXD-like motif is required for activity, no GGT activity in LH1 and LH2 | Homo sapiens | ? | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | calf skin gelatin | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | calf skin gelatin | Caenorhabditis elegans | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Caenorhabditis elegans | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 | Homo sapiens |
More | GGT activity is associated with lysyl hydroxylase, LH | Caenorhabditis elegans |
More | cf. EC 2.4.1.50 | Homo sapiens |
More | cf. EC 2.4.1.50 | Caenorhabditis elegans |