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Literature summary for 2.4.1.66 extracted from

  • Rautavuoma, K.; Takaluoma, K.; Passoja, K.; Pirskanen, A.; Kvist, A.P.; Kivirikko, K.I.; Myllyharju, J.
    Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1-3: The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity (2002), J. Biol. Chem., 277, 23084-23091.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length LH3 and the N-terminal 30 kDa fragment A with GGT activity is cloned, sequenced and expressed in High Five insect cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km: 4 mg/ml, denatured citrate soluble rat skin collagen Homo sapiens
0.15
-
UDP-glucose recombinant LH3/GGT Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
LH3 with GGT activity, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens there must be an additional glucosyltransferase to LH3 that is responsible for most of the collagen glucosylation in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information structural domain fragment A of LH3 with GGT activity contains 2 N-glycosylation sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant LH3/GGT/GT and N-terminal 30kDa fragment A of LH3 with GGT activity expressed in High Five insect cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase, collagen glucosyltransferase and galactosyltransferase activities Homo sapiens ?
-
?
additional information GGT and GT activities reside in the N-terminal 30.4 kDa structural domain fragment A of LH3, which plays no role in hydroxylase activity, only trace amounts of GT activity of LH3 Homo sapiens ?
-
?
additional information there must be an additional glucosyltransferase to LH3 that is responsible for most of the collagen glucosylation in vivo Homo sapiens ?
-
?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 80000-85000, recombinant LH3 with GGT/GT activities, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
More GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 Homo sapiens