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Literature summary for 2.4.1.66 extracted from
- Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes (1982), Methods Enzymol., 82, 245-304.
General Stability
| General Stability | Organism |
|---|---|
| concentrated enzyme, above 0.2 mg/ml, loses most of the activity | Gallus gallus |
| stable in crude tissue extracts | Gallus gallus |
| stable in crude tissue extracts | Cavia porcellus |
| stable in crude tissue extracts | Homo sapiens |
| stable in crude tissue extracts | Rattus norvegicus |
| stable in crude tissue extracts | Bos taurus |
| stable to repeated thawing and freezing, human liver or serum enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | Km: 0.5-1.0 mg/ml, denatured citrate soluble rat skin collagen | Rattus norvegicus | |
| additional information | - |
additional information | Km: 7-14 mg/ml, gelatinized calf skin collagen | Gallus gallus | |
| 0.003 | 0.006 | D-galactosylhydroxylysine | galactosylhydroxylysyl acceptor sites | Gallus gallus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Gallus gallus | - |
- |
| soluble | - |
Cavia porcellus | - |
- |
| soluble | - |
Homo sapiens | - |
- |
| soluble | - |
Rattus norvegicus | - |
- |
| soluble | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Gallus gallus | |
| Co2+ | - |
Cavia porcellus | |
| Co2+ | - |
Homo sapiens | |
| Co2+ | - |
Rattus norvegicus | |
| Co2+ | - |
Bos taurus | |
| Fe2+ | - |
Cavia porcellus | |
| Fe2+ | - |
Homo sapiens | |
| Fe2+ | - |
Rattus norvegicus | |
| Fe2+ | - |
Bos taurus | |
| Fe2+ | activation | Gallus gallus | |
| Fe2+ | also in the presence of Mn2+, at low concentration | Gallus gallus | |
| Mn2+ | requirement | Gallus gallus | |
| Mn2+ | requirement | Cavia porcellus | |
| Mn2+ | requirement | Homo sapiens | |
| Mn2+ | requirement | Rattus norvegicus | |
| Mn2+ | requirement | Bos taurus | |
| Mn2+ | activates, 2 Mn2+-ions bound per enzyme molecule, mechanism | Gallus gallus | |
| Mn2+ | activates, 2 Mn2+-ions bound per enzyme molecule, mechanism | Cavia porcellus | |
| Mn2+ | activates, 2 Mn2+-ions bound per enzyme molecule, mechanism | Homo sapiens | |
| Mn2+ | activates, 2 Mn2+-ions bound per enzyme molecule, mechanism | Rattus norvegicus | |
| Mn2+ | activates, 2 Mn2+-ions bound per enzyme molecule, mechanism | Bos taurus | |
| Mn2+ | 0.2-2.0 mM | Gallus gallus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Gallus gallus | involved in collagen biosynthesis | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Cavia porcellus | involved in collagen biosynthesis | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | involved in collagen biosynthesis | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Rattus norvegicus | involved in collagen biosynthesis | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Bos taurus | involved in collagen biosynthesis | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Rattus norvegicus | posttranslational modification of collagen | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | Homo sapiens | together with EC 2.4.1.50 involved in biosynthesis of glucosylgalactosylhydroxylysine units in collagens, basement membranes and certain serum glycoproteins | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Cavia porcellus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Gallus gallus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| 39800fold from embryo | Gallus gallus |
| partial | Rattus norvegicus |
| partial | Bos taurus |
| skin, partial, solubilized with Triton X-100 | Cavia porcellus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-glucose + [procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine = UDP + [procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine | reaction mechanism | Gallus gallus | |
| UDP-alpha-D-glucose + [procollagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine = UDP + [procollagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine | reaction mechanism | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood platelet | - |
Homo sapiens | - |
| bone | embryo bone | Rattus norvegicus | - |
| cartilage | embryonic | Gallus gallus | - |
| cartilage | embryonic | Cavia porcellus | - |
| embryo | - |
Gallus gallus | - |
| embryo | - |
Cavia porcellus | - |
| embryo | embryo bone | Rattus norvegicus | - |
| fibroblast | cultured skin fibroblasts | Homo sapiens | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Rattus norvegicus | - |
| macrophage | peritoneal | Rattus norvegicus | - |
| plasma | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
| skin | - |
Cavia porcellus | - |
| skin | cultured skin fibroblasts and skin extract | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.57 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme concentration 0.1 mg/ml, several months, 33% loss of activity | Gallus gallus |
| 4°C, enzyme concentration 0.1 mg/ml, 1 week, 33% loss of activity | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Cavia porcellus | ? | - |
? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | no substrates: hydroxylysyl- or glucosylgalactosyl hydroxylysyl-residues | Gallus gallus | ? | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | highly specific | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | highly specific | Cavia porcellus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | highly specific | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | highly specific | Rattus norvegicus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | highly specific | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acceptor specificity | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acceptor specificity | Cavia porcellus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acceptor specificity | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acceptor specificity | Rattus norvegicus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | acceptor specificity | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | ordered binding of Mn2+, UDP-glucose and collagen at low Mn2+ concentration and of two Mn2+, UDP-glucose and collagen at high Mn2+ concentration | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | heat-denatured citrate-soluble rat skin collagen | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | heat-denatured citrate-soluble rat skin collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | heat-denatured gelatinized calf skin collagen | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | heat-denatured gelatinized calf skin collagen | Rattus norvegicus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | absolute requirement: free epsilon-amino group in hydroxylysyl-residues | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | absolute requirement: free epsilon-amino group in hydroxylysyl-residues | Cavia porcellus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | absolute requirement: free epsilon-amino group in hydroxylysyl-residues | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | absolute requirement: free epsilon-amino group in hydroxylysyl-residues | Rattus norvegicus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | absolute requirement: free epsilon-amino group in hydroxylysyl-residues | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | involved in collagen biosynthesis | Gallus gallus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | involved in collagen biosynthesis | Cavia porcellus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | involved in collagen biosynthesis | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | involved in collagen biosynthesis | Rattus norvegicus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | involved in collagen biosynthesis | Bos taurus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | posttranslational modification of collagen | Rattus norvegicus | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen | together with EC 2.4.1.50 involved in biosynthesis of glucosylgalactosylhydroxylysine units in collagens, basement membranes and certain serum glycoproteins | Homo sapiens | UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen | - |
? | |
| UDP-glucose + D-galactosylhydroxylysine | in vitro | Gallus gallus | UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? | |
| UDP-glucose + D-galactosylhydroxylysine | in vitro | Homo sapiens | UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine | - |
? | |
| UDP-glucose + D-galactosylsphingosine | in vitro | Gallus gallus | UDP + 1,2-D-glucosyl-D-galactosylsphingosine | - |
? | |
| UDP-glucose + D-galactosylsphingosine | good acceptor | Gallus gallus | UDP + 1,2-D-glucosyl-D-galactosylsphingosine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72000-78000, value depends on the gel composition, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
| 37 | - |
assay at | Cavia porcellus |
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Bos taurus |
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