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Literature summary for 2.4.1.65 extracted from

  • Ma, B.; Wang, G.; Palcic, M.M.; Hazes, B.; Taylor, D.E.
    C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer (2003), J. Biol. Chem., 278, 21893-21900.
    View publication on PubMed
Search for more literature for 2.4.1.65

Cloned(Commentary)

Cloned (Comment) Organism
cloning of chimeric FucTs, expression in Escherichia coli HMS174DE3 cells Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0357
-
 GDP-fucose with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, strain UA948 Helicobacter pylori
0.048
-
 GDP-fucose with Gal-beta-1,4beta-GlcNAc-O-(CH2)8CO2CH3, wild type, strain NCTC116639 Helicobacter pylori
0.0481
-
 GDP-fucose with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(345DNPFIFC) Helicobacter pylori
0.0546
-
 GDP-fucose with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori
0.0683
-
 GDP-fucose with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, strain UA948 Helicobacter pylori
0.153
-
 GDP-fucose with beta-Gal1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT 11639(1-359)UA948(361-462) Helicobacter pylori
0.17
-
 Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT UA948(345DNPFIFC) Helicobacter pylori
0.188
-
 GDP-fucose with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori
0.213
-
 GDP-fucose with Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(345DNPFIFC) Helicobacter pylori
0.236
-
 GDP-fucose with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori
0.244
-
 GDP-fucose with Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3, chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori
0.31
-
 Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 wild type, strain NCTC116639 Helicobacter pylori
0.7
-
 Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT 11639(1-359)UA948(361-462) Helicobacter pylori
1.2
-
 Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 strain UA948 Helicobacter pylori
1.3
-
 Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori
2.2
-
 Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori
8.4
-
 Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 strain UA948 Helicobacter pylori
9.7
-
 Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori
20.8
-
 Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori
22.7
-
 Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 chimeric FucT UA948(345DNPFIFC) Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54600
-
 SDS-PAGE Helicobacter pylori
56000
-
 SDS-PAGE Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori Q9L8S4
-
-
Helicobacter pylori Q9L8S4 chimeric FucT 11639(1-359)UA948(361-462)
-
Helicobacter pylori Q9L8S4 chimeric FucT 11639(347CNDAHYSALH)
-
Helicobacter pylori Q9L8S4 chimeric FucT UA948(1-360)11639(360-478)
-
Helicobacter pylori Q9L8S4 chimeric FucT UA948(345DNPFIFC)
-
Helicobacter pylori NCTC116639 Q9L8S4
-
-
Helicobacter pylori UA948 Q9L8S4
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0163
-
 alpha 1,3 activity, wild type, strain NCTC116639 Helicobacter pylori
0.0238
-
 alpha-1,3 activity, alpha-1,4 activity is one third of the alpha 1,3-activity Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT UA948(345DNPFIFC) Helicobacter pylori GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, strain UA948 Helicobacter pylori GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori NCTC116639 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori NCTC116639 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT UA948(345DNPFIFC) Helicobacter pylori NCTC116639 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, strain UA948 Helicobacter pylori NCTC116639 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori UA948 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori UA948 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, chimeric FucT UA948(345DNPFIFC) Helicobacter pylori UA948 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,3-GlcNAc-O-(CH2)8CO2CH3 type 1 reaction, strain UA948 Helicobacter pylori UA948 GDP + Gal-beta-1,3[alpha-fucosyl(1,4)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, chimeric FucT 11639(1-359)UA948(361-462) Helicobacter pylori GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, chimeric FucT 11639(347CNDAHYSALH) Helicobacter pylori GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, chimeric FucT UA948(1-360)11639(360-478) Helicobacter pylori GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, chimeric FucT UA948(345DNPFIFC) Helicobacter pylori GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, strain NCTC116639 Helicobacter pylori GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, strain UA948 Helicobacter pylori GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, chimeric FucT 11639(1-359)UA948(361-462) Helicobacter pylori NCTC116639 GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?
GDP-fucose + Gal-beta-1,4-GlcNAc-O-(CH2)8CO2CH3 type 2 reaction, chimeric FucT 11639(1-359)UA948(361-462) Helicobacter pylori UA948 GDP + Gal-beta-1,4[alpha-fucosyl(1,3)]-GLCNAc-O-(CH2)8CO2CH3
-
 ?

Synonyms

Synonyms Comment Organism
alpha 1,3/4 fucosyltransferase
-
 Helicobacter pylori
FucT
-
 Helicobacter pylori