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Literature summary for 2.4.1.65 extracted from
- Effect of the manganese ion on human alpha3/4 fucosyltransferase III activity (2004), BioMetals, 17, 35-43.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Spodoptera frugiperda (Sf9) insect cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and presence of Mn2+ | Homo sapiens |  |
| 0.9 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and absence of Mn2+ | Homo sapiens | |
| 1.5 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and presence of Mn2+ | Homo sapiens | |
| 3.3 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and absence of Mn2+ | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | leads to 2.1fold activation of SFT3 | Homo sapiens | |
| Co2+ | leads to 2.8fold activation of SFT3 | Homo sapiens | |
| Cu2+ | no significant activity is detected | Homo sapiens | |
| Mg2+ | leads to 2.5fold activation of SFT3 | Homo sapiens | |
| Mn2+ | binds the enzyme and increases affinity for the acceptor. One possible functional role of manganese in catalysis can be as an electrophilic catalyst, co-ordinating the negative charges of the phosphate groups of the GDP-Fuc donor and promoting Fuc transfer. A low pH values such role would be played by the proton. Mn2+ leads to 2.7fold activation of SFT3 | Homo sapiens | |
| additional information | the enzyme retains approximately 35% of its maximal activity in the absence of metal ions | Homo sapiens | |
| Zn2+ | leads to 80% inhibition of STF3 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange-chromatography on a CM-Sepharose column, followed by an affinity chromatography on a GDP Fractogel column | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-fucose + Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | - |
Homo sapiens | GDP + Gal-beta-1,3[alpha-L-fucosyl-(1,4)]GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-3/4 fucosyltransferase III | - |
Homo sapiens |
| SFT3 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and absence of Mn2+ | Homo sapiens | |
| 0.2 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and presence of Mn2+ | Homo sapiens | |
| 0.2 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 7.0 and presence of Mn2+ | Homo sapiens | |
| 0.3 | - |
Gal-beta-1,3GlcNAcO(CH2)3NHCO(CH2)5NH-biotin | at pH 4.7 and absence of Mn2+ | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
in the absence of Mn2+ | Homo sapiens |
| 7 | - |
in the presence of Mn2+ | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | in the presence of Mn2+ | Homo sapiens |
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