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Literature summary for 2.4.1.65 extracted from

  • Dupuy, F.; Petit, J.M.; Mollicone, R.; Oriol, R.; Julien, R.; Maftah, A.
    A single amino acid in the hypervariable stem domain of vertebrate alpha1,3/1,4-fucosyltransferases determines the type 1/type 2 transfer. Characterization of acceptor substrate specificity of the Lewis enzyme by site-directed mutagenesis (1999), J. Biol. Chem., 274, 12257-12262.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D112E muation decreases activity of the enzyme and does not interfere with H-type 1/H-type 2 acceptors Homo sapiens
W111R the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors Homo sapiens
W11R/D112E the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors. Increased type 2 activity compared to mutant W111R Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
GDP-L-fucose mutant enzyme W111R Homo sapiens
0.032
-
GDP-L-fucose mutant enzyme W111R/D112E Homo sapiens
0.033
-
GDP-L-fucose mutant enzyme D112E Homo sapiens
0.035
-
GDP-L-fucose wild-type enzyme Homo sapiens
0.2
-
Fucalpha(1,2)Galbeta(1,3)GlcNAc wild-type enzyme Homo sapiens
0.4
-
Fucalpha(1,2)Galbeta(1,3)GlcNAc mutant enzyme D112E Homo sapiens
0.5
-
Fucalpha(1,2)Galbeta(1,4)GlcNAc mutant enzyme W111R/D112E Homo sapiens
0.7
-
Fucalpha(1,2)Galbeta(1,4)GlcNAc mutant enzyme W111R Homo sapiens
2
-
Fucalpha(1,2)Galbeta(1,3)GlcNAc mutant enzyme W111R/D112E Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
fucosyltransferase III
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc activity of the wild-type enzyme is about 3% of the activity with Fucalpha(1,2)Galbeta(1,4)GlcNAc. The mutation W111R shows higher activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc than for Fucalpha(1,2)Galbeta(1,4)GlcNAc. The addition mutation in W11R/D112E increases activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc compared to mutant W111R Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc
-
Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
?