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Literature summary for 2.4.1.65 extracted from

  • Vo, L.; Lee, S.; Marcinko, M.C.; Holmes, E.H.; Macher, B.A.
    Human alpha1,3/4-fucosyltransferases II. A single amino acid at the COOH terminus of FucT III and V alters their kinetic properties (1998), J. Biol. Chem., 273, 25250-25255.
    View publication on PubMed
Search for more literature for 2.4.1.65

Protein Variants

Protein Variants Comment Organism
D336A fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0336
-
 GDP-L-fucose fucosyltransferase III Homo sapiens
0.1
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc
-
 Homo sapiens
0.1145
-
 GDP-D-fucose mutant enzyme D336A Homo sapiens
3.8
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc fucosyltransferase III mutant enzyme D336A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 fucosyltransferase III
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-D-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc
-
 Homo sapiens ?
-
 ?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,3)GlcNAc
-
 Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
 ?
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc mutant enzyme D336A shows 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?
GDP-L-fucose + Galbeta(1,3)GlcNAc
-
 Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.02
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc fucosyltransferase III mutant enzyme D336A Homo sapiens
8.02
-
 Fucalpha(1,2)Galbeta(1,3)GlcNAc fucosyltransferase III Homo sapiens