Protein Variants | Comment | Organism |
---|---|---|
L649G | the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme | Caldanaerobacter subterraneus |
L649G/A693Q | the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme | Caldanaerobacter subterraneus |
L649G/A693Q/W371Y | the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme but displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product | Caldanaerobacter subterraneus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
beta-D-galactose 1-phosphate | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
0.7 | - |
beta-D-glucose 1-phosphate | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
3.1 | - |
beta-D-glucose 1-phosphate | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
3.7 | - |
D-glucose | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
3.8 | - |
beta-D-galactose 1-phosphate | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
4.6 | - |
D-glucose | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
58 | - |
D-galactose | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
125 | - |
D-galactose | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + beta-D-glucose 1-phosphate | Caldanaerobacter subterraneus | - |
lactotrehalose + phosphate | - |
r | |
lactotrehalose + phosphate | Caldanaerobacter subterraneus | - |
D-galactose + beta-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate | while the wild type enzyme shows very low activity, the mutant enzyme L649G/A693Q/W371Y displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product | Caldanaerobacter subterraneus | D-glucose + beta-D-galactose 1-phosphate | - |
r | |
D-galactose + beta-D-glucose 1-phosphate | - |
Caldanaerobacter subterraneus | lactotrehalose + phosphate | - |
r | |
D-glucose + beta-D-galactose 1-phosphate | - |
Caldanaerobacter subterraneus | alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate | - |
r | |
lactotrehalose + phosphate | - |
Caldanaerobacter subterraneus | D-galactose + beta-D-glucose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
trehalose phosphorylase | - |
Caldanaerobacter subterraneus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
D-galactose | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
1.2 | - |
beta-D-galactose 1-phosphate | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
1.6 | - |
beta-D-galactose 1-phosphate | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
4.2 | - |
D-glucose | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
7.4 | - |
beta-D-glucose 1-phosphate | mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
86 | - |
D-glucose | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
113 | - |
D-galactose | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus | |
114 | - |
beta-D-glucose 1-phosphate | wild type enzyme, at pH 6.0 and 60°C | Caldanaerobacter subterraneus |