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Literature summary for 2.4.1.63 extracted from
- The biosynthesis of cyanogenic glycosides in higher plants. I. Purification and properties of a uridine diphosphate-glucose-ketone cyanohydrin beta-glucosyltransferase from Linum usitatissimum L (1970), J. Biol. Chem., 245, 917-922.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for 2-mercaptoethanol or dithiothreitol, 0.01-100 mM | Linum usitatissimum |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes during purification | Linum usitatissimum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| MnCl2 | - |
Linum usitatissimum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for Mg2+ | Linum usitatissimum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + acetone cyanohydrin | Linum usitatissimum | pathway in cyanogenic glucoside biosynthesis | UDP + linamarin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Linum usitatissimum | - |
flax | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MnCl2, ammonium sulfate, DEAE-cellulose, Sephadex G-100 | Linum usitatissimum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Linum usitatissimum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0238 | - |
- |
Linum usitatissimum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| glycerol, ethanol, ammonium sulfate, DTT or albumin does not stabilize during storage at -18°C or room temperature | Linum usitatissimum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + 2-hydroxy-2-ethylbutanenitrile | - |
Linum usitatissimum | UDP + 2-(beta-D-glucopyranosyloxy)-2-ethylbutanenitrile | - |
? | |
| UDPglucose + 2-hydroxy-2-methylbutanenitrile | - |
Linum usitatissimum | UDP + 2-(beta-D-glucopyranosyloxy)-2-methylbutanenitrile | - |
? | |
| UDPglucose + 2-hydroxy-2-methylpropanenitrile | i.e. acetone cyanohydrin, the cyanohydrins of butanone and pentan-3-one may also be substrates, strict donor specificity, no activity with ADPglucose, CDPglucose, GDPglucose, IDPglucose or TDPglucose | Linum usitatissimum | UDP + linamarin | - |
? | |
| UDPglucose + acetone cyanohydrin | pathway in cyanogenic glucoside biosynthesis | Linum usitatissimum | UDP + linamarin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Linum usitatissimum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Linum usitatissimum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9.3 | approx. half-maximal activity at pH 7.2 and 9.3, due to substrate stability, Tris buffer is preferred over glycine-NaOH buffer | Linum usitatissimum |
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Release 2023.1 (January 2023)
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