Any feedback?
Literature summary for 2.4.1.58 extracted from
- Biosynthesis of bacterial lipopolysaccharide. II. Incorporation of glucose and galactose catalyzed by particulate and soluble enzymes in Salmonella (1964), J. Biol. Chem., 239, 2788-2795.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing: rapid loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
lipopolysaccharide | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 0.074 | - |
UDPglucose | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | most of the activity is located in the cell-wall fraction, but also significant activity in soluble fraction | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | soluble enzyme: MgCl2 stimulates more than MnCl2, optimal concentration: 0.02 M | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | soluble enzyme: MgCl2 stimulates more than MnCl2 | Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | involved in biosynthesis of cell wall lipopolysaccharide | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium SL797 | involved in biosynthesis of cell wall lipopolysaccharide | UDP + D-glucosyllipopolysaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium SL797 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C or 4°C, soluble enzyme, overnight storage, 30-50% loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium SL797 | ? | - |
? | |
| UDPglucose + lipopolysaccharide | UDPglucose is the only effective glucosyl donor | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | transfers glucosyl residues to the backbone portion of lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | involved in biosynthesis of cell wall lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | UDPglucose is the only effective glucosyl donor | Salmonella enterica subsp. enterica serovar Typhimurium SL797 | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | transfers glucosyl residues to the backbone portion of lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium SL797 | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium SL797 | UDP + D-glucosyllipopolysaccharide | - |
? | |
| UDPglucose + lipopolysaccharide | involved in biosynthesis of cell wall lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium SL797 | UDP + D-glucosyllipopolysaccharide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
soluble enzyme, Tris buffer | Salmonella enterica subsp. enterica serovar Typhimurium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
