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Literature summary for 2.4.1.56 extracted from
- High-level expression of the Neisseria meningitidis lgtA gene in Escherichia coli and characterization of the encoded N-acetylglucosaminyltransferase as a useful catalyst in the synthesis of GlcNAcb1->3Gal and GalNAcb1->3Gal linkages (1999), Glycobiology, 9, 1061-1071.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme promise to be a useful catalyst in the preparation of both GlcNAcbeta1-3Gal and GalNAc1-3Gal linkages | Neisseria meningitidis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and overexpression of lgtA gene encoding enzyme in Escherichia coli BL21(DE3)/pLys, polypeptide of 333 amino acids | Neisseria meningitidis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by UMP | Neisseria meningitidis | |
| UDP | 1.5 mM: 50% inhibition | Neisseria meningitidis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
UDP-N-acetyl-D-glucosamine | - |
Neisseria meningitidis | |
| 2.4 | - |
UDP-N-acetyl-D-galactosamine | - |
Neisseria meningitidis | |
| 4.3 | - |
Galbeta1-4Glcbeta-O-4-nitrophenol | UDP-N-acetyl-D-glucosamine as donor | Neisseria meningitidis | |
| 4.5 | - |
Galbeta1-4Glcbeta-O-4-nitrophenol lipooligosaccharide | UDP-N-acetyl-D-galactosamine as donor | Neisseria meningitidis | |
| 6.1 | - |
Galbeta1-4GlcNAcbeta-O-4-nitrophenol lipooligosaccharide | UDP-N-acetyl-D-glucosamine as donor | Neisseria meningitidis | |
| 7.2 | - |
Galbeta1-4GlcNAcbeta-O-4-nitrophenol lipooligosaccharide | UDP-N-acetyl-D-galactosamine as donor | Neisseria meningitidis | |
| 27 | - |
Galbeta1-3GalNAcbeta-O-4-nitrophenol | UDP-N-acetyl-D-glucosamine as donor | Neisseria meningitidis | |
| 28 | - |
Galbeta1-4Glc lipooligosaccharide | UDP-N-acetyl-D-glucosamine as donor | Neisseria meningitidis | |
| 36 | - |
Galbeta1-4Glc lipooligosaccharide | UDP-N-acetyl-D-galactosamine as donor | Neisseria meningitidis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | activates, less effective than Mn2+ | Neisseria meningitidis | |
| Mg2+ | activates, less effective than Mn2+ | Neisseria meningitidis | |
| Mn2+ | absolute requirement for divalent cations of which Mn2+ is most active, optimal concentration: 15 mM | Neisseria meningitidis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + lipooligosacccharide | Neisseria meningitidis | involved in the synthesis of the lacto-N-neo-tetraose structural element of the bacterial lipooligosacccharide, lactose is the natural acceptor disaccharide sequence in lipooligosaccharide | UDP + N-acetyl-D-glucosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + lipooligosacccharide | Neisseria meningitidis H44/76 | involved in the synthesis of the lacto-N-neo-tetraose structural element of the bacterial lipooligosacccharide, lactose is the natural acceptor disaccharide sequence in lipooligosaccharide | UDP + N-acetyl-D-glucosaminyllipooligosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neisseria meningitidis | - |
immunotype L3 | - |
| Neisseria meningitidis H44/76 | - |
immunotype L3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, recombinant enzyme expressed in Escherichia coli | Neisseria meningitidis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | 0.09 | - |
Neisseria meningitidis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% v/v glycerol, 5mg/ml bovine serum albumin, at least 5 months, stable | Neisseria meningitidis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no sugar donors: UDPglucose, UDPgalactose, UDPglucosamine, overview over oligosaccharide acceptor specificity | Neisseria meningitidis | ? | - |
? | |
| additional information | no sugar donors: UDPglucose, UDPgalactose, UDPglucosamine, overview over oligosaccharide acceptor specificity | Neisseria meningitidis H44/76 | ? | - |
? | |
| UDP-N-acetyl-D-galactosamine + lipooligosacccharide | 76% of activity with UDP-N-acetyl-D-glucosamine, 10fold lower affinity than for UDP-N-acetyl-D-glucosamine, beta1-3-linkage of N-acetyl-D-galactosamine to accepting Gal residues | Neisseria meningitidis | UDP + N-acetyl-D-galactosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + lipooligosacccharide | 76% of activity with UDP-N-acetyl-D-glucosamine, 10fold lower affinity than for UDP-N-acetyl-D-glucosamine, beta1-3-linkage of N-acetyl-D-galactosamine to accepting Gal residues | Neisseria meningitidis H44/76 | UDP + N-acetyl-D-galactosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-3GalNAcbeta-O-4-nitrophenol | - |
Neisseria meningitidis | UDP + GlcNAcbeta-1-3Galbeta1-3GalNAcbeta-O-4-nitrophenol | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-3GalNAcbeta-O-p-nitrophenol | low affinity | Neisseria meningitidis | UDP + GlcNAcbeta-1-3Galbeta1-3GalNAcbeta-O-p-nitrophenol | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-4Glc lipooligosaccharide | low affinity | Neisseria meningitidis | UDP + GlcNAcbeta1-3Galbeta1-4Glc lipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-4Glcbeta-O-4-nitrophenol | - |
Neisseria meningitidis | UDP + GlcNAcbeta1-3Galbeta1-4Glcbeta-O-4-nitrophenol | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-4Glcbeta-O-4-nitrophenol lipooligosaccharide | - |
Neisseria meningitidis | UDP + GlcNAcbeta1-3Galbeta1-4Glcbeta-O-4-nitrophenol lipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-4Glcbeta-O-p-nitrophenol | - |
Neisseria meningitidis | UDP + GlcNAcbeta1-3Galbeta1-4Glcbeta-O-p-nitrophenol | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-4GlcNAcbeta-O-4-nitrophenol lipooligosaccharide | - |
Neisseria meningitidis | UDP + GlcNAcbeta1-3Galbeta1-4GlcNAcbeta-O-4-nitrophenol lipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta1-4GlcNAcbeta-O-p-nitrophenol | - |
Neisseria meningitidis | UDP + GlcNAcbeta1-3-Galbeta1-4GlcNAcbeta-O-p-nitrophenol | - |
? | |
| UDP-N-acetyl-D-glucosamine + lactose | lactose is the highly preferred acceptor substrate | Neisseria meningitidis | UDP + beta-D-GlcNAc-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc | - |
? | |
| UDP-N-acetyl-D-glucosamine + lactose | lactose is the highly preferred acceptor substrate | Neisseria meningitidis H44/76 | UDP + beta-D-GlcNAc-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc | - |
? | |
| UDP-N-acetyl-D-glucosamine + lipooligosacccharide | involved in the synthesis of the lacto-N-neo-tetraose structural element of the bacterial lipooligosacccharide, terminal Gal is an absolute acceptor requirement, broad acceptor specificity toward both alpha- and beta-galactosides structurally related to N- or O-protein or lipid-linked oligosaccharides, beta1-3-linkage of N-acetyl-D-glucosamine to accepting Gal residues in the extending lipooligosacccharide, lactose is the highly preferred acceptor substrate, enzyme also acts efficiently on monomeric and dimeric N-acetyllactosamine | Neisseria meningitidis | UDP + N-acetyl-D-glucosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + lipooligosacccharide | involved in the synthesis of the lacto-N-neo-tetraose structural element of the bacterial lipooligosacccharide, lactose is the natural acceptor disaccharide sequence in lipooligosaccharide | Neisseria meningitidis | UDP + N-acetyl-D-glucosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + lipooligosacccharide | involved in the synthesis of the lacto-N-neo-tetraose structural element of the bacterial lipooligosacccharide, terminal Gal is an absolute acceptor requirement, broad acceptor specificity toward both alpha- and beta-galactosides structurally related to N- or O-protein or lipid-linked oligosaccharides, beta1-3-linkage of N-acetyl-D-glucosamine to accepting Gal residues in the extending lipooligosacccharide, lactose is the highly preferred acceptor substrate, enzyme also acts efficiently on monomeric and dimeric N-acetyllactosamine | Neisseria meningitidis H44/76 | UDP + N-acetyl-D-glucosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + lipooligosacccharide | involved in the synthesis of the lacto-N-neo-tetraose structural element of the bacterial lipooligosacccharide, lactose is the natural acceptor disaccharide sequence in lipooligosaccharide | Neisseria meningitidis H44/76 | UDP + N-acetyl-D-glucosaminyllipooligosaccharide | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyllactosamine | enzyme also acts efficiently on monomeric and dimeric N-acetyllactosamine | Neisseria meningitidis | UDP + N-acetyl-D-glucosaminyl-N-acetyllactosamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | - |
Neisseria meningitidis |
| 30 | - |
assay at | Neisseria meningitidis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Neisseria meningitidis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
broad pH-range within enzyme is active | Neisseria meningitidis |
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