KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
calf skin collagen | value based on actual amount of galactosylhydroxylysyl-residues in calf skin collagen | Homo sapiens | |
0.049 | - |
UDPgalactose | calf skin collagen as acceptor | Homo sapiens | |
0.28 | - |
ichthyocol | value based on actual amount of galactosylhydroxylysyl-residues in ichthyocol | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, 60% as effective as Mn2+ | Homo sapiens | |
Mg2+ | activates, 20% as effective as Mn2+ | Homo sapiens | |
Mn2+ | requirement | Homo sapiens | |
Mn2+ | optimal concentration: 5-10 mM | Homo sapiens | |
additional information | not activated by Cu2+ | Homo sapiens | |
additional information | not activated by Zn2+ | Homo sapiens | |
additional information | not activated by Co2+ | Homo sapiens | |
additional information | not activated by Ni2+ | Homo sapiens | |
additional information | not activated by Cd2+ | Homo sapiens | |
additional information | not activated by Fe2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | intracellular post-translational modification in collagen biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, solubilized with Triton X-100 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Homo sapiens | - |
fetus | - |
Homo sapiens | - |
IMR-90 cell | fetal lung diploid fibroblasts, cell lines WI-38 and IMR-90 | Homo sapiens | - |
WI-38 cell | fetal lung diploid fibroblasts, WI-38 and IMR-90 cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no acceptor: thyroglobulin unit B | Homo sapiens | ? | - |
? | |
additional information | no acceptor: free hydroxylysine | Homo sapiens | ? | - |
? | |
additional information | intracellular post-translational modification in collagen biosynthesis | Homo sapiens | ? | - |
? | |
UDP-alpha-D-galactose + calf skin collagen | - |
Homo sapiens | ? | - |
? | |
UDPgalactose + ichthyocol | fish collagen ichthyocol as best substrate, peptides derived from collagenase- and pronase-digestion of ichthyocol are less effective | Homo sapiens | ? | - |
? | |
UDPgalactose + procollagen 5-hydroxy-L-lysine | acid-soluble calf skin collagen | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
UDPgalactose + procollagen 5-hydroxy-L-lysine | fish collagen ichthyocol as best substrate, peptides derived from collagenase- and pronase-digestion of ichthyocol are less effective, native collagens from swim bladder or calf skin are more efficient substrates than collagen glycopeptides | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
UDPgalactose + procollagen 5-hydroxy-L-lysine | bovine glomerular basement membranes with carbohydrate units removed, native less effective | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? | |
UDPgalactose + procollagen 5-hydroxy-L-lysine | high specificity for specific hydroxylysine residues in collagen | Homo sapiens | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | 2 pH-optima: 6-6.5 and 7.5-8 | Homo sapiens |
7.5 | 8 | 2 pH-optima: 6-6.5 and 7.5-8 | Homo sapiens |