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Literature summary for 2.4.1.50 extracted from

  • Kivirikko, K.I.; Myllylä, R.
    Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes (1982), Methods Enzymol., 82, 245-304.
    View publication on PubMed

General Stability

General Stability Organism
unstable during purification Gallus gallus
unstable in crude extracts Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
concanavalin A
-
Gallus gallus
UDP
-
Gallus gallus
UDP
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Cavia porcellus
additional information
-
additional information Km: 15-35 mg/ml, gelatinized calf skin collagen Gallus gallus
additional information
-
additional information Km: 2-4 mg/ml, denatured citrate-soluble rat skin collagen Gallus gallus
0.1 0.2 hydroxylysyl acceptor sites
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Gallus gallus 5783
-
endoplasmic reticulum
-
Cavia porcellus 5783
-
endoplasmic reticulum
-
Homo sapiens 5783
-
endoplasmic reticulum
-
Rattus norvegicus 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Cavia porcellus
Mn2+
-
Rattus norvegicus
Mn2+ requirement Gallus gallus
Mn2+ requirement Homo sapiens
Mn2+ optimal concentration: 2 mM Gallus gallus
additional information
-
Cavia porcellus
additional information
-
Homo sapiens
additional information requires a bivalent cation Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPgalactose + procollagen 5-hydroxy-L-lysine Gallus gallus involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine Cavia porcellus involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine Homo sapiens involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine Rattus norvegicus involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Gallus gallus
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein probably a glycoprotein Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
1000fold Gallus gallus
partial Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
Homo sapiens
-
bone embryo bone Rattus norvegicus
-
embryo
-
Gallus gallus
-
embryo
-
Cavia porcellus
-
embryo
-
Homo sapiens
-
embryo embryo bone Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
skin
-
Cavia porcellus
-
skin cultured fibroblasts Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0147
-
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no acceptor: galactosylhydroxylysine Gallus gallus ?
-
?
additional information no acceptor: free hydroxylysine Gallus gallus ?
-
?
additional information no acceptor: free hydroxylysine Cavia porcellus ?
-
?
additional information no acceptor: free hydroxylysine Homo sapiens ?
-
?
additional information no acceptor: free hydroxylysine Rattus norvegicus ?
-
?
UDP-alpha-D-galactose + hydroxylysyl acceptor sites
-
Gallus gallus ?
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine
-
Homo sapiens UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine
-
Rattus norvegicus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine triple helix conformation of native collagen at 30°C prevents glycosylation Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine guinea pig skin collagen with sugar residues removed Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine guinea pig skin collagen with sugar residues removed Cavia porcellus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine bovine renal glomerular basement membrane Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine bovine anterior lens capsule collagen with carbohydrate units removed Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine gelatinized insoluble calf skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine acceptor specificity: requires free epsilon-amino group in hydroxylysyl-residues Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine high specificity for galactosyl acceptor Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine high specificity for galactosyl acceptor Cavia porcellus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine denatured citrat-soluble rat skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement Cavia porcellus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement Homo sapiens UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine involved in collagen biosynthesis, post-translational modification, probably involved in synthesis of carbohydrate units in complement Rattus norvegicus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus
37
-
assay at Cavia porcellus
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.4
-
Gallus gallus