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Literature summary for 2.4.1.5 extracted from

  • Baruah, R.; Deka, B.; Goyal, A.
    Purification and characterization of dextransucrase from Weissella cibaria RBA12 and its application in in vitro synthesis of prebiotic oligosaccharides in mango and pineapple juiceEntrapment of purified novel dextransucrase obtained from newly isolated Acetobacter tropicalis and its comparative study of kinetic parameters with free enzymes (2017), LWT-Food Sci. Technol., 84, 449-456 .
No PubMed abstract available
Search for more literature for 2.4.1.5

Application

Application Comment Organism
food industry the enzyme catalzes the in vitro synthesis of prebiotic oligosaccharides in mango and pineapple juices. Sucrose content of the juices is eliminated resulting in its lower calorific value. Potential of dextransucrase for production of functional foods Weissella cibaria

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 1 mM, 29% decrease of activity Weissella cibaria
EDTA 0.5 mM, 31% decrease of activity Weissella cibaria
MnSO4 1 mM, 22% decrease of activity Weissella cibaria
NiSO4 1 mM, 36% decrease of activity Weissella cibaria
Urea
-
 Weissella cibaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19.2
-
 sucrose pH 5.4, 40°C Weissella cibaria

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Weissella cibaria
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 3 mM, enhances activity by 25% Weissella cibaria
Mg2+ 0.6 mM, enhances activity by 40% Weissella cibaria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 SDS-PAGE Weissella cibaria

Organism

Organism UniProt Comment Textmining
Weissella cibaria
-
-
-
Weissella cibaria RBA12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
25fold purification by PEG-400 and PEG-1500 fractionation, followed by gel filtration Weissella cibaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + D-glucose isomalto-oligosaccharides from DP3 to DP5 along with isomaltose 44 (DP2) and leucrose (DP2) are synthesized Weissella cibaria D-fructose + isomalto-oligosaccharide + isomaltose + leucrose
-
 ?
sucrose + D-glucose isomalto-oligosaccharides from DP3 to DP5 along with isomaltose 44 (DP2) and leucrose (DP2) are synthesized Weissella cibaria RBA12 D-fructose + isomalto-oligosaccharide + isomaltose + leucrose
-
 ?

Subunits

Subunits Comment Organism
? x * 180000, SDS-PAGE Weissella cibaria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Weissella cibaria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 1 h, pH 5.4, stable Weissella cibaria
40
-
 1 h, pH 5.4, 8% loss of activity Weissella cibaria
60
-
 1 h, pH 5.4, complete loss of activity Weissella cibaria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
 Weissella cibaria

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 1 h, 32% loss of activity Weissella cibaria
5
-
 1 h, 12% loss of activity Weissella cibaria
5.5
-
 1 h, 7% loss of activity Weissella cibaria
6
-
 1 h, 17% loss of activity Weissella cibaria