Literature summary for 2.4.1.5 extracted from

Florez Guzman, G.Y.; Hurtado, G.B.; Ospina, S.A.
New dextransucrase purification process of the enzyme produced by Leuconostoc mesenteroides IBUN 91.2.98 based on binding product and dextranase hydrolysis (2018), J. Biotechnol., 265, 8-14 .

Inhibitors

Inhibitors	Comment	Organism
Al3+	2 mM, 99% inhibition	Leuconostoc mesenteroides
Fe3+	2 mM, 97% inhibition	Leuconostoc mesenteroides
sucrose	substrate inhibition at concentrations over 584 mM sucrose	Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
584	-	sucrose	pH 5, 30°C	Leuconostoc mesenteroides

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Leuconostoc mesenteroides	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	2 mM, 27% increase in activity	Leuconostoc mesenteroides
Mg2+	2 mM, 37% increase in activity	Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
170100	-	calculation from sequence	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	-	-	-
Leuconostoc mesenteroides IBUN 91.2.98	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300 kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography. The obtained enzyme has a purification factor of 118 and a yield of 26% from the initial extract	Leuconostoc mesenteroides

Purification (Comment)

Organism

purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300 kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography. The obtained enzyme has a purification factor of 118 and a yield of 26% from the initial extract

Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
335.1	-	pH 5, 30°C	Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose	the produced dextran has a molecular size of 800-1000 kDa	Leuconostoc mesenteroides	D-fructose + dextran	-	?
sucrose	the produced dextran has a molecular size of 800-1000 kDa	Leuconostoc mesenteroides IBUN 91.2.98	D-fructose + dextran	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3	-	about 25% loss of activity after 20 min, about 45% loss of activity after 45 min, about 55% loss of activity after 80 min	Leuconostoc mesenteroides
10	-	about 35% loss of activity after 20 min, about 35% loss of activity after 45 min, about 40% loss of activity after 80 min	Leuconostoc mesenteroides
57	-	80 min, stable	Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Leuconostoc mesenteroides

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	7	pH 4.0: 49% of maximal activity, pH 7.0: 25% of maximal activity	Leuconostoc mesenteroides

pI Value

Organism	Comment	pI Value Maximum	pI Value
Leuconostoc mesenteroides	calculation from sequence	-	4.2