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Literature summary for 2.4.1.5 extracted from

  • Shukla, S.; Verma, A.; Kajala, I.; Nyyssolae, A.; Baruah, R.; Katina, K.; Juvonen, R.; Tenkanen, M.; Goyal, A.
    Structure modelling and functional analysis of recombinant extransucrase from Weissella confusa Cab3 expressed in Lactococcus lactis (2016), Prep. Biochem. Biotechnol., 46, 822-832.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant soluble extracellular enzyme expression in Lactococcus lactis Weissella confusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2
-
[(1->6)-alpha-D-glucosyl]n pH 5.0, 35°C Weissella confusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
x * 180000, recombinant enzyme, SDS-PAGE Weissella confusa

Organism

Organism UniProt Comment Textmining
Weissella confusa X4QKX0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme 4fold from Lactococcus lactis by PEG fractionation Weissella confusa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
partially purrified recombinant enzyme, pH 5.0, 35°C Weissella confusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Subunits

Subunits Comment Organism
? x * 180000, recombinant enzyme, SDS-PAGE Weissella confusa
More the enzyme structure contains the domains A, B, C, IV, and V, structure superposition, overview Weissella confusa

Synonyms

Synonyms Comment Organism
Cab3
-
Weissella confusa
DSR
-
Weissella confusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
recombinant enzyme Weissella confusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
recombinant enzyme Weissella confusa

General Information

General Information Comment Organism
additional information structure modeling using the crystal structure of the enzyme from Lactobacillus reuteri, PDB ID 3HZ3, and Streptococcus mutans, PDB ID 3AlB, as templates. Asp510 acts as a nucleophile, Glu548 acts as catalytic acid/base, and Asp621 acts a stransition state stabilizer Weissella confusa