Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 64% inhibition at 10 mM | Weissella cibaria | |
Urea | inactivation at 5 M | Weissella cibaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Weissella cibaria | |
13 | - |
[(1->6)-alpha-D-glucosyl]n | pH 5.4, 35°C | Weissella cibaria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates at 2 mM | Weissella cibaria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
177000 | - |
x * 177000, SDS-PAGE | Weissella cibaria |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | Weissella cibaria | production of dextran | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Weissella cibaria JAG8 | production of dextran | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella cibaria | B9UNL6 | isolated from apple, gene dsrWC | - |
Weissella cibaria JAG8 | B9UNL6 | isolated from apple, gene dsrWC | - |
Purification (Comment) | Organism |
---|---|
native enzyme 37fold by PEG400 fractionation and gel filtration | Weissella cibaria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
partially purrified recombinant enzyme, pH 5.4, 35°C | Weissella cibaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | production of dextran | Weissella cibaria | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | production of dextran, optimal at 5% w/v sucrose | Weissella cibaria | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | production of dextran | Weissella cibaria JAG8 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | production of dextran, optimal at 5% w/v sucrose | Weissella cibaria JAG8 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 177000, SDS-PAGE | Weissella cibaria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Weissella cibaria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
purified enzyme, pH 5.4, 10-500 mM sodium acetate buffer, 1 h, stable | Weissella cibaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Weissella cibaria |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
purified enzyme, 35°C, 10-500 mM sodium acetate buffer, 1 h, stable | Weissella cibaria |