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Literature summary for 2.4.1.5 extracted from

  • Zhang, H.; Gan, W.; Zhang, Y.; Hu, X.
    Synthesis of isomalto-oligosaccharides by using recombinant dextransucrase and Hypocrea lixii dextranase (2013), J. Chem. Pharm. Res., 5, 49-57.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli strain BL21 (DE3)/pET28-dexYG Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synergistic catalytic manner of recombinant dextransucrase and Hypocrea lixii dextranase in production of isomalto-oligosaccharides, produced by sucrose conversion and dextran hydrolysis, molecular weight change of dextrans, overview. High sucrose concentrations in the dextransucrase and dextranase system lead to accumulation of oligodextrans with molecular weights below 15 kDa. Combined usage of recombinant dextransucrase and Hypocrea lixii dextranase produces isomalto-oligosaccharides with a degree of polymerization of 2-10 Leuconostoc mesenteroides ?
-
?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
Leuconostoc mesenteroides