General Stability | Organism |
---|---|
enzyme deactivation follows first order rate kinetics | Leuconostoc mesenteroides |
glutaraldehyde does not support the stability, rather it inhibits the enzyme activity, also glycerol, PEG 8000, and dextran (500 kDa) do not stabilize the enzyme | Leuconostoc mesenteroides |
Tween 80 stabilizes the purified enzyme, maximally at 30°C | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 50% inhibition at 1 mM | Leuconostoc mesenteroides | |
Glutaraldehyde | does not support enzyme stability, but inhibits the enzyme activity | Leuconostoc mesenteroides | |
Urea | denatures the enzyme and causes 45%, 90% and 98% loss of activity within 30 min when treated at 1 M, 3 M, and 5 M concentration, respectively | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme deactivation follows fi rst order rate kinetics | Leuconostoc mesenteroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | activates slightly by 8% at 1 mM | Leuconostoc mesenteroides | |
CoCl2 | activates 22% at 4 mM | Leuconostoc mesenteroides | |
MgCl2 | activates slightly by 6% at 1 mM | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides NRRL B-640 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by PEG 1500 fractionation | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23 | - |
purified enzyme, pH 5.4, 30°C | Leuconostoc mesenteroides |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, loss of 25% activity within 14 days at 30C | Leuconostoc mesenteroides |
4°C, purified enzyme, loss of 92% activity within 4 days at 30°C | Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | purified enzyme, stable at, rapid loss of activity above. Loss of 65% activity within 20 h at 30°C without added stabilizer, in presence of Tween 80 the enzyme loses only 8% activity. Loss of 92% activity at 30°C within 4 days | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
assay at | Leuconostoc mesenteroides |