Cloned (Comment) | Organism |
---|---|
gene dsrWC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain Top10F' and expression in Escherichia coli strain BL21(DE3) | Weissella cibaria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Weissella cibaria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
161998 | - |
x * 161998, sequence calculation | Weissella cibaria |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1,6)-alpha-D-glucosyl]n | Weissella cibaria | - |
D-fructose + [(1,6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella cibaria | B9UNL6 | isolated from human saliva, gene dsrWC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 5.1fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Weissella cibaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant DSRWC synthesizes oligosaccharides in the presence of maltose or isomaltose as an acceptor and the synthesized products include alpha-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue. rDSRWC synthesizes water-soluble polymers using sucrose as substrate, and rDSRBWC synthesizes dextran and leucrose [alpha-D-glucopyranosyl-(1,5)-beta-D-fructofuranose] | Weissella cibaria | ? | - |
? | |
sucrose + [(1,6)-alpha-D-glucosyl]n | - |
Weissella cibaria | D-fructose + [(1,6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1,6)-alpha-D-glucosyl]n | In the presence of maltose, the most abundant reaction product is panose, i.e. MP1 or alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-D-glucopyranose, with 35.3%, detailed NMR product identification and structure analysis, overview | Weissella cibaria | D-fructose + [(1,6)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 161998, sequence calculation | Weissella cibaria |
Synonyms | Comment | Organism |
---|---|---|
DSRWC | - |
Weissella cibaria |
glucansucrase | - |
Weissella cibaria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Weissella cibaria |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 62% at 20°C, activity range | Weissella cibaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Weissella cibaria |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | activity range | Weissella cibaria |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | over 80% of maximal activity within this range, but the activity decreases rapidly outside this pH range | Weissella cibaria |