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Literature summary for 2.4.1.5 extracted from

  • Majumder, A.; Purama, R.K.; Goyal, A.
    An overview of purification methods of glycoside hydrolase family 70 dextransucrase (2007), Indian J. Microbiol., 47, 197-206.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme from strain B-512F in Escherichia coli Leuconostoc mesenteroides

General Stability

General Stability Organism
the enzyme can be stabilized by high-molecular-weight dextran, polyethyleneglycol or nonionic detergents such as Tween 80 Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-trinitrobenzene-sulphonic acid inactivation Leuconostoc mesenteroides
Cu2+
-
Leuconostoc mesenteroides
diethyldicarbonate
-
Leuconostoc mesenteroides
EDTA inactivation, reversible by Ca2+ Leuconostoc mesenteroides
Fe3+
-
Leuconostoc mesenteroides
Mn2+
-
Leuconostoc mesenteroides
o-phthalaldehyde inactivation Leuconostoc mesenteroides
pyridoxal-5'-phosphate inactivation Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
sucrose 30°C Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides B-512F
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography. Various methods such as precipitation by ammonium sulphate, ethanol, or polyethylene glycol, phase partitioning, ultrafiltration and chromatography are used to purify the enzyme, detailed overview. Purification of dextransucrase is rendered difficult by the presence of viscous dextran in the medium Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glucose transfer from sucrose to dextrans leading to polymerization with release of D-fructose, the enzyme contains an active site His residue as well as an essential lysine residue Leuconostoc mesenteroides ?
-
?
additional information glucose transfer from sucrose to dextrans leading to polymerization with release of D-fructose, the enzyme contains an active site His residue as well as an essential lysine residue Leuconostoc mesenteroides B-512F ?
-
?
sucrose + (1,6-alpha-D-glucosyl)n
-
Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
?
sucrose + (1,6-alpha-D-glucosyl)n
-
Leuconostoc mesenteroides B-512F D-fructose + (1,6-alpha-D-glucosyl)n+1
-
?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the glycoside hydrolase family 70 Leuconostoc mesenteroides
sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Leuconostoc mesenteroides