Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
S455K | produces a glucan with 85% 6-linked glucopyranosyl residues | Leuconostoc mesenteroides |
T350K | produces a glucan with 85% 6-linked glucopyranosyl residues | Leuconostoc mesenteroides |
T350K/S455K | mutant enzyme exhibits a 10fold increase in glucosyltransferase activity over those of the parental DSRS-His6 and its T350K and S455K mutants | Leuconostoc mesenteroides |
T350K/S455K | produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
NRRL B-512F | - |
Leuconostoc mesenteroides NRRL B-512F | - |
NRRL B-512F | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (1,6-alpha-D-glucosyl)n | native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues. Mutant enzyme T350K and S455K produce a glucan with 85% 6-linked glucopyranosyl residues. The mutant T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues | Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
sucrose + (1,6-alpha-D-glucosyl)n | native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues. Mutant enzyme T350K and S455K produce a glucan with 85% 6-linked glucopyranosyl residues. The mutant T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues | Leuconostoc mesenteroides NRRL B-512F | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DSRS | - |
Leuconostoc mesenteroides |