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Literature summary for 2.4.1.5 extracted from
- Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299 (1976), J. Biochem., 79, 1301-1308.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dextrans | activity of enzyme N is more effectively stimulated than enzyme I | Leuconostoc mesenteroides |  |
General Stability
| General Stability | Organism |
|---|---|
| maximal stabilization of soluble enzyme by 5 mM Ca2+ | Leuconostoc mesenteroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, stimulates activity of enzyme N, enzyme I is inhibited | Leuconostoc mesenteroides | |
| Co2+ | 1 mM, stimulates activity of enzyme N, enzyme I is inhibited | Leuconostoc mesenteroides | |
| EDTA | enzyme I not affected, enzyme N inhibited, inhibition overcome by Ca2+ | Leuconostoc mesenteroides | |
| Fe2+ | 1 mM, stimulates activity of enzyme N, enzyme I is inhibited | Leuconostoc mesenteroides | |
| Guanidine-HCl | 0.025 M, 65% inhibition of enzyme N, no effect on enzyme I | Leuconostoc mesenteroides | |
| Mg2+ | 1 mM, stimulates activity of enzyme N, enzyme I is inhibited | Leuconostoc mesenteroides | |
| Mn2+ | enzyme I and N, strong inhibition | Leuconostoc mesenteroides | |
| phenylmercuric acetate | - |
Leuconostoc mesenteroides | |
| SDS | - |
Leuconostoc mesenteroides | |
| Urea | 0.25 M, enzyme N completely inactivated, enzyme I retains 60% of its activity | Leuconostoc mesenteroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effect of temperature on KM-value | Leuconostoc mesenteroides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Leuconostoc mesenteroides | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity of enzyme N | Leuconostoc mesenteroides | |
| Co2+ | 1 mM, stimulates activity of enzyme N, enzyme I is inhibited | Leuconostoc mesenteroides | |
| Fe2+ | 1 mM, stimulates activity of enzyme N | Leuconostoc mesenteroides | |
| Mg2+ | 1 mM, stimulates activity of enzyme N | Leuconostoc mesenteroides | |
| additional information | 2 enzyme forms: I and N, enzyme I is gradually converted into enzyme N upon ageing, conversion is stimulated in the presence of NaCl | Leuconostoc mesenteroides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, enzyme I, SDS-PAGE | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
NRRL B-1299 | - |
| Leuconostoc mesenteroides NRRL B-1299 | - |
NRRL B-1299 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 forms: I and N, enzyme I is gradually converted into enzyme N upon ageing, conversion is stimulated in the presence of NaCl | Leuconostoc mesenteroides |
| NRRL B-1299 | Leuconostoc mesenteroides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Leuconostoc mesenteroides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides NRRL B-1299 | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, enzyme I, SDS-PAGE | Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
enyme N | Leuconostoc mesenteroides |
| 40 | 45 | enzyme I | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
10 min, enzyme I and N stable | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
enzyme N | Leuconostoc mesenteroides |
| 6 | - |
enzyme I | Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
4°C, 24 h, enzyme N is stable | Leuconostoc mesenteroides |
| 6 | - |
4°C, 24 h, enzyme I is stable | Leuconostoc mesenteroides |
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