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Literature summary for 2.4.1.49 extracted from

  • O'Neill, E.C.; Pergolizzi, G.; Stevenson, C.E.M.; Lawson, D.M.; Nepogodiev, S.A.; Field, R.A.
    Cellodextrin phosphorylase from Ruminiclostridium thermocellum X-ray crystal structure and substrate specificity analysis (2017), Carbohydr. Res., 451, 118-132 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme, selenomethionine-labelled enzyme, or enzyme in complex with cellohexaose and phosphate, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 300 mM KCl, 100 mM HEPES pH 7.5 Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
(1->4)-beta-GlcN(Glc)2
-
 Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 [(1->4)-beta-D-glucosyl]5 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
0.54
-
 [(1->4)-beta-D-glucosyl]4 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
0.68
-
 [(1->4)-beta-D-glucosyl]3 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
1.9
-
 [(1->4)-beta-D-glucosyl]6 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
2.6
-
 [(1->4)-beta-D-glucosyl]2 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
24
-
 phenyl beta-D-glucopyranoside apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-beta-D-glucosyl]n + phosphate Acetivibrio thermocellus
-
 [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-beta-D-glucosyl]n + phosphate Acetivibrio thermocellus YM4
-
 [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate Acetivibrio thermocellus
-
 [(1->4)-beta-D-glucosyl]n + phosphate
-
 r
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate Acetivibrio thermocellus YM4
-
 [(1->4)-beta-D-glucosyl]n + phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus Q93HT8
-
-
Acetivibrio thermocellus YM4 Q93HT8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex S75 gel filtration Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellobiose + alpha-D-glucose 1-phosphate weak acceptor Acetivibrio thermocellus ?
-
 ?
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellobiose + alpha-D-glucose 1-phosphate weak acceptor Acetivibrio thermocellus YM4 ?
-
 ?
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellohexaose + alpha-D-glucose 1-phosphate good acceptor Acetivibrio thermocellus ?
-
 ?
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellohexaose + alpha-D-glucose 1-phosphate good acceptor Acetivibrio thermocellus YM4 ?
-
 ?
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellotriose + alpha-D-glucose 1-phosphate good acceptor, the enzyme is able to extend 8-aminopyrene-1,3,6-trisulfonic acid-labelled cellotriose to oligomers up to DP 16 Acetivibrio thermocellus ?
-
 ?
additional information no activity with D-glucose or [(1->4)-beta-D-mannosyl]3 Acetivibrio thermocellus ?
-
-
additional information no activity with D-glucose or [(1->4)-beta-D-mannosyl]3 Acetivibrio thermocellus YM4 ?
-
-
phenyl beta-D-glucopyranoside + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus ?
-
 r
[(1->4)-beta-D-glucosyl]2 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]3 + phosphate
-
 r
[(1->4)-beta-D-glucosyl]3 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]4 + phosphate
-
 r
[(1->4)-beta-D-glucosyl]4 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]5 + phosphate
-
 r
[(1->4)-beta-D-glucosyl]5 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]6 + phosphate
-
 r
[(1->4)-beta-D-glucosyl]6 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]7 + phosphate
-
 r
[(1->4)-beta-D-glucosyl]n + phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-beta-D-glucosyl]n + phosphate
-
 Acetivibrio thermocellus YM4 [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-glucosyl]n + phosphate
-
 r
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus YM4 [(1->4)-beta-D-glucosyl]n + phosphate
-
 r
[(1->4)-beta-D-xylosyl]3 + alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus [(1->4)-beta-D-xylosyl]4 + phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 114364, calculated from amino acid sequence Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
CDP
-
 Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 [(1->4)-beta-D-glucosyl]5 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
5
-
 [(1->4)-beta-D-glucosyl]4 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
7.6
-
 [(1->4)-beta-D-glucosyl]6 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
9.5
-
 [(1->4)-beta-D-glucosyl]3 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
15
-
 phenyl beta-D-glucopyranoside apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
17
-
 [(1->4)-beta-D-glucosyl]2 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.63
-
 phenyl beta-D-glucopyranoside apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
4
-
 [(1->4)-beta-D-glucosyl]6 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
6.5
-
 [(1->4)-beta-D-glucosyl]2 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
9.3
-
 [(1->4)-beta-D-glucosyl]4 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
12
-
 [(1->4)-beta-D-glucosyl]5 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus
14
-
 [(1->4)-beta-D-glucosyl]3 apparent value, at pH 7.5 and 21°C Acetivibrio thermocellus