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Literature summary for 2.4.1.49 extracted from

  • Nakai, H.; Hachem, M.A.; Petersen, B.O.; Westphal, Y.; Mannerstedt, K.; Baumann, M.J.; Dilokpimol, A.; Schols, H.A.; Duus, J.O.; Svensson, B.
    Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum (2010), Biochimie, 92, 1818-1826.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21(DE3) Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
2 * 115000, SDS-PAGE, gel filtration Acetivibrio thermocellus
218000
-
gel filtration Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DJQ6
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.071
-
substrate cellobiose, pH 5.7, 40°C Acetivibrio thermocellus
8.7
-
substrate cellotriose, pH 5.7, 40°C Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + alpha-D-glucose 1-phosphate glucose, cellotriose, sophorose and laminaribinose are further substrates; sophorose, laminaribiose, gentibiose and chitobiose are not phosphorolysed; alpha-N-acetyl D-glucosamine 1-phosphate, alpha-D-mannose 1-phosphate and alpha-D-galactose 1-phosphate are not donors Acetivibrio thermocellus cellotriose + phosphate
-
r
cellobiose + alpha-D-glucosyl-1-fluoride
-
Acetivibrio thermocellus cellotriose + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 115000, SDS-PAGE, gel filtration Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
cellodextrin phosphorylase
-
Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
15 min Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
reverse phosphorolysis reaction Acetivibrio thermocellus
5.7
-
phosphorolytic activity Acetivibrio thermocellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.6 7 4°C, 24 h Acetivibrio thermocellus