Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
2 * 115000, SDS-PAGE, gel filtration | Acetivibrio thermocellus |
218000 | - |
gel filtration | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DJQ6 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography | Acetivibrio thermocellus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.071 | - |
substrate cellobiose, pH 5.7, 40°C | Acetivibrio thermocellus |
8.7 | - |
substrate cellotriose, pH 5.7, 40°C | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + alpha-D-glucose 1-phosphate | glucose, cellotriose, sophorose and laminaribinose are further substrates; sophorose, laminaribiose, gentibiose and chitobiose are not phosphorolysed; alpha-N-acetyl D-glucosamine 1-phosphate, alpha-D-mannose 1-phosphate and alpha-D-galactose 1-phosphate are not donors | Acetivibrio thermocellus | cellotriose + phosphate | - |
r | |
cellobiose + alpha-D-glucosyl-1-fluoride | - |
Acetivibrio thermocellus | cellotriose + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 115000, SDS-PAGE, gel filtration | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
cellodextrin phosphorylase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
reverse phosphorolysis reaction | Acetivibrio thermocellus |
5.7 | - |
phosphorolytic activity | Acetivibrio thermocellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.6 | 7 | 4°C, 24 h | Acetivibrio thermocellus |