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Literature summary for 2.4.1.49 extracted from

  • Reichenbecher, M.; Lottspeich, F.; Bronnenmeier, K.
    Purification and properties of a cellobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic thermophile Clostridium stercorarium (1997), Eur. J. Biochem., 247, 262-267.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis Thermoclostridium stercorarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
cellotriose
-
Thermoclostridium stercorarium
0.05
-
cellotetraose
-
Thermoclostridium stercorarium
0.17
-
cellopentaose
-
Thermoclostridium stercorarium
0.19
-
cellohexaose
-
Thermoclostridium stercorarium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
N-terminal amino acid sequence Thermoclostridium stercorarium
91000
-
gel filtration Thermoclostridium stercorarium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1,4-beta-D-glucosyl)n + phosphate Thermoclostridium stercorarium involved in the catabolism of cellulose (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
?
(1,4-beta-D-glucosyl)n + phosphate Thermoclostridium stercorarium NCIB 11754 involved in the catabolism of cellulose (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermoclostridium stercorarium P77846
-
-
Thermoclostridium stercorarium P77846 gene CepB
-
Thermoclostridium stercorarium NCIB 11754 P77846
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoclostridium stercorarium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.2
-
purified enzyme, assay at 60°C and pH 6.0 Thermoclostridium stercorarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-beta-D-glucosyl)n + phosphate specific for oligosaccharides Thermoclostridium stercorarium (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
?
(1,4-beta-D-glucosyl)n + phosphate involved in the catabolism of cellulose Thermoclostridium stercorarium (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
?
(1,4-beta-D-glucosyl)n + phosphate specific for oligosaccharides Thermoclostridium stercorarium NCIB 11754 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
?
(1,4-beta-D-glucosyl)n + phosphate involved in the catabolism of cellulose Thermoclostridium stercorarium NCIB 11754 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
?
additional information no phosphorolysis activity with cellobiose Thermoclostridium stercorarium ?
-
?
additional information no phosphorolysis activity with cellobiose Thermoclostridium stercorarium NCIB 11754 ?
-
?
phosphate + cellohexaose
-
Thermoclostridium stercorarium cellopentaose + glucose 1-phosphate
-
?
phosphate + cellopentaose
-
Thermoclostridium stercorarium cellotetraose + glucose 1-phosphate
-
?
phosphate + cellotetraose
-
Thermoclostridium stercorarium cellotriose + glucose 1-phosphate
-
?
phosphate + cellotetraose
-
Thermoclostridium stercorarium NCIB 11754 cellotriose + glucose 1-phosphate
-
?
phosphate + cellotriose
-
Thermoclostridium stercorarium cellobiose + glucose 1-phosphate
-
?
phosphate + cellotriose
-
Thermoclostridium stercorarium NCIB 11754 cellobiose + glucose 1-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 91000, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermoclostridium stercorarium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life: 30 h Thermoclostridium stercorarium
65
-
inactivation within 20 h Thermoclostridium stercorarium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
cellotriose
-
Thermoclostridium stercorarium
6.1
-
cellohexaose
-
Thermoclostridium stercorarium
6.6
-
cellopentaose
-
Thermoclostridium stercorarium
6.9
-
cellotetraose
-
Thermoclostridium stercorarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Thermoclostridium stercorarium

pH Range

pH Minimum pH Maximum Comment Organism
5 8 half-maximal activity at pH 5.0 and pH 8.0 Thermoclostridium stercorarium