Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Thermoclostridium stercorarium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
cellotriose | - |
Thermoclostridium stercorarium | |
0.05 | - |
cellotetraose | - |
Thermoclostridium stercorarium | |
0.17 | - |
cellopentaose | - |
Thermoclostridium stercorarium | |
0.19 | - |
cellohexaose | - |
Thermoclostridium stercorarium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
N-terminal amino acid sequence | Thermoclostridium stercorarium |
91000 | - |
gel filtration | Thermoclostridium stercorarium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-beta-D-glucosyl)n + phosphate | Thermoclostridium stercorarium | involved in the catabolism of cellulose | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
(1,4-beta-D-glucosyl)n + phosphate | Thermoclostridium stercorarium NCIB 11754 | involved in the catabolism of cellulose | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoclostridium stercorarium | P77846 | - |
- |
Thermoclostridium stercorarium | P77846 | gene CepB | - |
Thermoclostridium stercorarium NCIB 11754 | P77846 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoclostridium stercorarium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.2 | - |
purified enzyme, assay at 60°C and pH 6.0 | Thermoclostridium stercorarium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-beta-D-glucosyl)n + phosphate | specific for oligosaccharides | Thermoclostridium stercorarium | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
(1,4-beta-D-glucosyl)n + phosphate | involved in the catabolism of cellulose | Thermoclostridium stercorarium | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
(1,4-beta-D-glucosyl)n + phosphate | specific for oligosaccharides | Thermoclostridium stercorarium NCIB 11754 | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
(1,4-beta-D-glucosyl)n + phosphate | involved in the catabolism of cellulose | Thermoclostridium stercorarium NCIB 11754 | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
additional information | no phosphorolysis activity with cellobiose | Thermoclostridium stercorarium | ? | - |
? | |
additional information | no phosphorolysis activity with cellobiose | Thermoclostridium stercorarium NCIB 11754 | ? | - |
? | |
phosphate + cellohexaose | - |
Thermoclostridium stercorarium | cellopentaose + glucose 1-phosphate | - |
? | |
phosphate + cellopentaose | - |
Thermoclostridium stercorarium | cellotetraose + glucose 1-phosphate | - |
? | |
phosphate + cellotetraose | - |
Thermoclostridium stercorarium | cellotriose + glucose 1-phosphate | - |
? | |
phosphate + cellotetraose | - |
Thermoclostridium stercorarium NCIB 11754 | cellotriose + glucose 1-phosphate | - |
? | |
phosphate + cellotriose | - |
Thermoclostridium stercorarium | cellobiose + glucose 1-phosphate | - |
? | |
phosphate + cellotriose | - |
Thermoclostridium stercorarium NCIB 11754 | cellobiose + glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 91000, SDS-PAGE | Thermoclostridium stercorarium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermoclostridium stercorarium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 30 h | Thermoclostridium stercorarium |
65 | - |
inactivation within 20 h | Thermoclostridium stercorarium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
cellotriose | - |
Thermoclostridium stercorarium | |
6.1 | - |
cellohexaose | - |
Thermoclostridium stercorarium | |
6.6 | - |
cellopentaose | - |
Thermoclostridium stercorarium | |
6.9 | - |
cellotetraose | - |
Thermoclostridium stercorarium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Thermoclostridium stercorarium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | half-maximal activity at pH 5.0 and pH 8.0 | Thermoclostridium stercorarium |