Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | required for activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
Phospholipid | effect on several strains, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability | Organism |
---|---|
one cycle of freezing and thawing causes marked loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Nonsubstrate lipopolysaccharide | competitive, Salmonella typhimurium SL1032, TV119, LT2 lipopolysaccharides; effect on several strains, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
UDP-galactose | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | no activity in absence of divalent cations, 20% of the activation with MgCl2 | Salmonella enterica subsp. enterica serovar Typhimurium | |
MgCl2 | no activity in absence of divalent cations, highest activity with MgCl2 | Salmonella enterica subsp. enterica serovar Typhimurium | |
MnCl2 | no activity in absence of divalent cations, 20% of the activation with MgCl2 | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
G-30A | - |
Salmonella enterica subsp. enterica serovar Typhimurium G-30A | - |
G-30A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | unidentified lipid-soluble component which contains no phosphorus is bound to the enzyme, it causes aggregation of the enzyme, but no role of the lipid in enzyme reaction has yet been established | Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
several bacterial strains, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
0.00048 | - |
supernatant fraction | Salmonella enterica subsp. enterica serovar Typhimurium |
0.0015 | - |
purified enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, pH 6.8, 0.02-0.04 mg/ml protein, stable for at least 1 month | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + lipopolysaccharide | lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060 | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | highly specific for UDPgalactose | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060 | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | highly specific for UDPgalactose | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
UDP-galactose + lipopolysaccharide | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
4°C, several days, inactivation | Salmonella enterica subsp. enterica serovar Typhimurium |