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Literature summary for 2.4.1.44 extracted from

  • Endo, A.; Rothfield, L.
    Studies of a phospholipid-requiring bacterial enzyme. I. Purification and properties of uridine diphosphate galactose: lipopolysaccharide alpha-3-galactosyl transferase (1969), Biochemistry, 8, 3500-3507.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid required for activity Salmonella enterica subsp. enterica serovar Typhimurium
Phospholipid effect on several strains, overview Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

General Stability Organism
one cycle of freezing and thawing causes marked loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
Nonsubstrate lipopolysaccharide competitive, Salmonella typhimurium SL1032, TV119, LT2 lipopolysaccharides; effect on several strains, overview Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
UDP-galactose
-
Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 no activity in absence of divalent cations, 20% of the activation with MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium
MgCl2 no activity in absence of divalent cations, highest activity with MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium
MnCl2 no activity in absence of divalent cations, 20% of the activation with MgCl2 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium G-30A transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium G-30A catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
G-30A
-
Salmonella enterica subsp. enterica serovar Typhimurium G-30A
-
G-30A
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information unidentified lipid-soluble component which contains no phosphorus is bound to the enzyme, it causes aggregation of the enzyme, but no role of the lipid in enzyme reaction has yet been established Salmonella enterica subsp. enterica serovar Typhimurium

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
several bacterial strains, overview Salmonella enterica subsp. enterica serovar Typhimurium
0.00048
-
supernatant fraction Salmonella enterica subsp. enterica serovar Typhimurium
0.0015
-
purified enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, pH 6.8, 0.02-0.04 mg/ml protein, stable for at least 1 month Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + lipopolysaccharide lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060 Salmonella enterica subsp. enterica serovar Typhimurium UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide highly specific for UDPgalactose Salmonella enterica subsp. enterica serovar Typhimurium UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism Salmonella enterica subsp. enterica serovar Typhimurium UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060 Salmonella enterica subsp. enterica serovar Typhimurium G-30A UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide highly specific for UDPgalactose Salmonella enterica subsp. enterica serovar Typhimurium G-30A UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium G-30A UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir
UDP-galactose + lipopolysaccharide catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism Salmonella enterica subsp. enterica serovar Typhimurium G-30A UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
4°C, several days, inactivation Salmonella enterica subsp. enterica serovar Typhimurium