Application | Comment | Organism |
---|---|---|
molecular biology | polyclonal rabbit anti-GalNAc-T14 IgG (1: 1000 in Western blot, titer: appr. 1: 16000) for study of expression and distribution of human GalNAc-T14 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and HEK-293T cells | Homo sapiens |
full-length GalNAc-T14 in pFlag-CMV2, expression in HEK293T cells | Homo sapiens |
full-length GalNAc-T14 in pGEX-4T-1, expression in Escherichia coli BL21(DE3) | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
endogenous GalNAc-T14 in HEK293T cells detected by anti-GalNAc-T14 antibody | Homo sapiens |
61000 | - |
Flag-tag fusion protein expressed in HEK293T cells detected by anti-Flag and anti-GalNAc-T14 antibody | Homo sapiens |
87000 | - |
SDS-PAGE | Homo sapiens |
87000 | - |
bacterially expressed GST-fusion protein (GST: 26 kDa) detected by anti-GST and anti-GalNAc-T14 antibody | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96FL9 | - |
- |
Homo sapiens | Q96FL9 | GalNAc-T14 | - |
Purification (Comment) | Organism |
---|---|
GST fusion protein purified by affinity chromatography, elution with 10 mM glutathione, pH8 | Homo sapiens |
GSTrap FF chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | cDNA library used for cloning | Homo sapiens | - |
HEK-293T cell | expression detected by Western blot | Homo sapiens | - |
MCF-7 cell | no expression detected by Western blot | Homo sapiens | - |
renal cell carcinoma cell | RCC 7860, expression detected by Western blot | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | - |
Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GalNAc-T | - |
Homo sapiens |
GalNAc-T14 | - |
Homo sapiens |
polypeptide N-acetylgalactosaminyltransferase | - |
Homo sapiens |
polypeptide N-acetylgalactosaminyltransferase 14 | - |
Homo sapiens |