Cloned (Comment) | Organism |
---|---|
PGANT35A in pUAST plasmid for trachea-specific, Gal4-iduceable expression in transgenic flies under control of breathless promoter | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of pgant35A mutant germ line clones arrests to 88% during embryogenesis, does not reach pupation | Drosophila melanogaster |
additional information | pgant35A mutant phenotype less severe and 7% rescue of lethality upon trachea-specific expression of PGANT35A wild-type | Drosophila melanogaster |
additional information | severity of phenotypes of transgenic flies correlates with molecular and biochemical characterization of the severity of the mutant allele | Drosophila melanogaster |
pgant35A-3775 | transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-HG8 allele, germ line clone: heterozygous with pgant35A-HG8 or pgant35A-SF32 allele, nonsense mutation results in translational stop and loss of enzymatic activity | Drosophila melanogaster |
pgant35A-HG8 | transgenic fly: heterozygous with pgant35A-SF32 or pgant35A-3775 phenotype, germ line clone: homozygous or with pgant35A-3775 allele , nonsense mutation results in translational stop and loss of enzymatic activity | Drosophila melanogaster |
pgant35A-SF32 | transgenic fly: heterozygous with pgant35A-3775 or pgant35A-HG8 phenotype, germ line clone: homozygous or with pgant35A-3775 allele, missense mutation: Arg to Trp, results in diminished activity | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Drosophila melanogaster | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q8MVS5 | PGANT35A; PGANT35A is an orthologue of mammalian ppGalNTase -T11 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic tracheal system | dorsal longitudinal tracheal trunks and tracheal transverse connectives at embryonic stages 15-17, loss of PGANT35A activity leads to tracheal defects at embryonic stages 15-17, altered intracellular trafficking, reduction in apical and luminal epithelial marker components, alterations in cell shape, polarity and zona adherens formation, mislocation and disfunction of septate junctions, disruption of paracellular diffusion barrier formation | Drosophila melanogaster | - |
hindgut | loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components | Drosophila melanogaster | - |
salivary gland | loss of PGANT35A activity does not alter morphology or staining patterns of epithelial marker components | Drosophila melanogaster | - |
trachea | loss of PGANT35A activity leads to diminished O-glycosylation in epithelial cells of tracheal tube as well as reduced amount of GalNAc alpha-S/T glycans at the apical cell surface | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-GalNAc + polypeptide | - |
Drosophila melanogaster | GalNAc-alpha-polypeptide + UDP | major mucin-type O-glycan in embryonic tracheal system along the apical region and luman of the tracheal tubes O-glycan, detectable by lectins Jacalin, soybean agglutinin and Vicia villosa agglutinin | ? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Drosophila melanogaster | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGANT | - |
Drosophila melanogaster |
ppGalNAcT | - |
Drosophila melanogaster |
ppGaNTase | - |
Drosophila melanogaster |
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase | - |
Drosophila melanogaster |