Cloned (Comment) | Organism |
---|---|
expression in COS7 cells as truncated version from pIMKF4 SV40 | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 10 mM MnCl2 | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Bos taurus | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Drosophila melanogaster | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q07537 | ppGalNAc T1 | - |
Drosophila melanogaster | Q6WV17 | PGANT5; PGANT5 is the orthologue of mammalian ppGalNAc T1 | - |
Drosophila melanogaster | Q6WV19 | PGANT2; PGANT2 is the orthologue of mammalian ppGalNAc T2 | - |
Homo sapiens | Q10471 | ppGalNAc T2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-GalNAc + GAGA(X)3T(X)3AGAGK | - |
Drosophila melanogaster | GAGA(X)3(GalNAc)T(X)3AGAGK + UDP | - |
? | |
UDP-GalNAc + GAGA(X)3T(X)3AGAGK | - |
Bos taurus | UDP + GAGA(X)3(GalNAc)T(X)3AGAGK | - |
? | |
UDP-GalNAc + GAGA(X)3T(X)3AGAGK | - |
Homo sapiens | UDP + GAGA(X)3(GalNAc)T(X)3AGAGK | - |
? | |
UDP-GalNAc + GAGA(X)5T(X)5AGAGK | transferase activity relatively insensitive to the nature of amino acids 4 and 5 residues away from site of glycosylation, high correlation between peptide preferences of PGANT5 and mammalian orthologue ppGalNAc T1 with r(square) = 0.67, the hydrophobic residue proline is preferred at the -1, +1, and +3 positions, the hydrophobic residues valine and tyrosin are moderately preferred at the -1 and +3 positions, the hydrophobic residue methionine is disfavoured at the -1 position, low hydrophilic preference at the -1 position, strong glutamate/aspartate preference at the +1 position, higher preference of glutamate at +1 and +2 positions than mammalian orthologue ppGalNAc T1 | Drosophila melanogaster | GAGA(X)5(GalNAc)T(X)5AGAGK + UDP | - |
? | |
UDP-GalNAc + GAGA(X)5T(X)5AGAGK | transferase activity relatively insensitive to the nature of amino acids 4 and 5 residues away from site of glycosylation, very high correlation between peptide preferences of PGANT2 and mammalian orthologue ppGalNAc T2 with r(square) = 0.92 | Drosophila melanogaster | GAGA(X)5(GalNAc)T(X)5AGAGK + UDP | - |
? | |
UDP-GalNAc + GAGA(X)5T(X)5AGAGK | very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92 | Homo sapiens | UDP + GAGA(X)5(GalNAc)T(X)5AGAGK | - |
? | |
UDP-GalNAc + GAGA(X)5T(X)5AGAGK | very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92, the hydrophobic residue proline is preferred at the -1, +1, and +3 positions, the hydrophobic residues valine and tyrosin are moderately preferred at the -1 and +3 positions, the hydrophobic residue methionine is disfavoured at the -1 position, low hydrophilic preference at the -1 position, strong glutamate/aspartate preference at the +1 position, lower preference of glutamate at +1 and +2 positions than fly orthologue PGANT5 | Bos taurus | UDP + GAGA(X)5(GalNAc)T(X)5AGAGK | - |
? | |
UDP-GalNAc + GAGA(X)nT(X)nAGAGK | library of unmodified peptides, with randomized residue at position X and n=3 or n=5 | Drosophila melanogaster | GAGA(X)n(GalNAc)T(X)nAGAGK + UDP | - |
? | |
UDP-GalNAc + GAGA(X)nT(X)nAGAGK | library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation | Bos taurus | GAGA(X)n(GalNAc)T(X)nAGAGK + UDP | - |
? | |
UDP-GalNAc + GAGA(X)nT(X)nAGAGK | library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation | Homo sapiens | GAGA(X)n(GalNAc)T(X)nAGAGK + UDP | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Drosophila melanogaster | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGANT | - |
Drosophila melanogaster |
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase | - |
Drosophila melanogaster |
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase | abbreviated as ppGalNAc T | Bos taurus |
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase | abbreviated as ppGalNAc T | Homo sapiens |