Application | Comment | Organism |
---|---|---|
drug development | further studies of the substrate specificity of these and potentially additional enzymes are required, but it may be anticipated that this understanding will be significant for insight into O-glycosylation processing and of practical use, for example, for designing appropriate mammalian expression systems for recombinant glycoproteins in drug use | Homo sapiens |
drug development | further studies of the substrate specificity of these and potentially additional enzymes are required, but it may be anticipated that this understanding will be significant for our insight into O-glycosylation processing and of practical use, for example, for designing appropriate mammalian expression systems for recombinant glycoproteins in drug use | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
PCR cloning of human version of GalNAc-T1, based on the reported sequence of bovine GalNAc-T1. Polymerase chain reaction cloning and sequencing of the human version of the bovine transferase are presented, and 98% similarity at the amino acid level is found | Homo sapiens |
PCR construct is expressed in insect cells using a baculovirus vector. Expression of GalNAc-T2 in Sf9 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
single band, SDS-PAGE. Polymerase chain reaction cloning and sequencing of the human version of the bovine transferase are presented, and 98% similarity at the amino acid level is found | Homo sapiens |
59210 | - |
molecular mass of the deduced amino acid sequence of the soluble transferase is 59205 Da, which is slightly higher than the experimentally determined molecular weight but within the limits of accuracy of the SDS-PAGE and gel filtration systems used | Homo sapiens |
64700 | - |
cDNA sequence has a 571-amino acid coding region indicating a protein of 64.7 kDa with a type II domain structure | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q10471 | - |
- |
Homo sapiens | Q10472 | - |
- |
Purification (Comment) | Organism |
---|---|
purified to apparent homogeneity using a synthetic acceptor peptide as affinity ligand | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
heart | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
kidney | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
liver | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
lung | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
MKN-45 cell | gastric cancer cell line, cDNA encoding the transferase is cloned and sequenced from a cDNA library of a human cancer cell line | Homo sapiens | - |
pancreas | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
placenta | - |
Homo sapiens | - |
placenta | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
skeletal muscle | Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.75 | - |
purification step 5, Mono S (NaCl) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRL | - |
Homo sapiens | UDP + N-actetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRL | - |
? | |
UDP-N-acetyl-D-galactosamine + PTTTPISTTMVTPTPTPTC | - |
Homo sapiens | UDP + N-actetyl-D-galactosaminylated PTTTPISTTMVTPTPTPTC | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GalNAc-T1 | data suggest that the purified human GalNAc-transferase is a novel member of a family of polypeptide GalNAc-transferases, and a nomenclature GalNAc-T1 and GalNAc-T2 is introduced to distinguish the members | Homo sapiens |
GalNAc-T2 | data suggest that the purified human GalNAc-transferase is a novel member of a family of polypeptide GalNAc-transferases, and a nomenclature GalNAc-T1 and GalNAc-T2 is introduced to distinguish the members | Homo sapiens |
GalNAc-transferase | - |
Homo sapiens |
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase | - |
Homo sapiens |