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Literature summary for 2.4.1.41 extracted from

  • White, T.; Bennett, E.P.; Takio, K.; Sorensen, T.; Bonding, N.; Clausen, H.
    Purification and cDNA cloning of a human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (1995), J. Biol. Chem., 270, 24156-24165.
    View publication on PubMed

Application

Application Comment Organism
drug development further studies of the substrate specificity of these and potentially additional enzymes are required, but it may be anticipated that this understanding will be significant for insight into O-glycosylation processing and of practical use, for example, for designing appropriate mammalian expression systems for recombinant glycoproteins in drug use Homo sapiens
drug development further studies of the substrate specificity of these and potentially additional enzymes are required, but it may be anticipated that this understanding will be significant for our insight into O-glycosylation processing and of practical use, for example, for designing appropriate mammalian expression systems for recombinant glycoproteins in drug use Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
PCR cloning of human version of GalNAc-T1, based on the reported sequence of bovine GalNAc-T1. Polymerase chain reaction cloning and sequencing of the human version of the bovine transferase are presented, and 98% similarity at the amino acid level is found Homo sapiens
PCR construct is expressed in insect cells using a baculovirus vector. Expression of GalNAc-T2 in Sf9 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
single band, SDS-PAGE. Polymerase chain reaction cloning and sequencing of the human version of the bovine transferase are presented, and 98% similarity at the amino acid level is found Homo sapiens
59210
-
molecular mass of the deduced amino acid sequence of the soluble transferase is 59205 Da, which is slightly higher than the experimentally determined molecular weight but within the limits of accuracy of the SDS-PAGE and gel filtration systems used Homo sapiens
64700
-
cDNA sequence has a 571-amino acid coding region indicating a protein of 64.7 kDa with a type II domain structure Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q10471
-
-
Homo sapiens Q10472
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to apparent homogeneity using a synthetic acceptor peptide as affinity ligand Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
heart Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
kidney Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
liver Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
lung Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
MKN-45 cell gastric cancer cell line, cDNA encoding the transferase is cloned and sequenced from a cDNA library of a human cancer cell line Homo sapiens
-
pancreas Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
placenta
-
Homo sapiens
-
placenta Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-
skeletal muscle Northern analysis of eight human tissues differ clearly from that of the bovine GalNAc-transferase Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.75
-
purification step 5, Mono S (NaCl) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRL
-
Homo sapiens UDP + N-actetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRL
-
?
UDP-N-acetyl-D-galactosamine + PTTTPISTTMVTPTPTPTC
-
Homo sapiens UDP + N-actetyl-D-galactosaminylated PTTTPISTTMVTPTPTPTC
-
?

Synonyms

Synonyms Comment Organism
GalNAc-T1 data suggest that the purified human GalNAc-transferase is a novel member of a family of polypeptide GalNAc-transferases, and a nomenclature GalNAc-T1 and GalNAc-T2 is introduced to distinguish the members Homo sapiens
GalNAc-T2 data suggest that the purified human GalNAc-transferase is a novel member of a family of polypeptide GalNAc-transferases, and a nomenclature GalNAc-T1 and GalNAc-T2 is introduced to distinguish the members Homo sapiens
GalNAc-transferase
-
Homo sapiens
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
-
Homo sapiens