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Literature summary for 2.4.1.41 extracted from

  • Homa, F.L.; Baker, C.A.; Thomsen, D.R.; Elhammer, A.P.
    Conversion of a bovine UDP-GalNAc:polypeptide, N-acetylgalactosaminyltransferase to a soluble, secreted enzyme, and expression in Sf9 cells (1995), Protein Expr. Purif., 6, 141-148.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA is cloned, a soluble enzyme construct prepared by substituting the honeybee melittin leader sequence for the sequences coding for the cytoplasmic and membrane spanning domains is expressed in insect Sf9 cells using a recombinant baculovirus, nucleotide and amino acid sequence Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
UDP-GalNAc
-
Bos taurus
3.6
-
Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg
-
Bos taurus
6.5
-
Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Bos taurus 16020
-
membrane type II membrane protein containing a 23-amino acid transmembrane domain and an 8-amino acid cytoplasmic tail Bos taurus 16020
-
additional information intracellular membrane-bound enzyme, localized in the secretory pathway Bos taurus
-
-
soluble soluble, secreted enzyme is prepared by substituting the honeybee melittin leader sequence for the sequences coding for the cytoplasmic and membrane spanning domains Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + polypeptide Bos taurus post-translational, initial reaction in O-linked oligosaccharide biosynthesis UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 3 N- and 1 O-linked oligosaccharide structures per molecule, soluble enzyme contains N-acetylglucosamine, N-acetylgalactosamine, mannose, galactose and fucose, N-linked structures are likely to be of the truncated high-mannose type Bos taurus

Purification (Commentary)

Purification (Comment) Organism
21fold, recombinant soluble enzyme, expressed in Sf9 cells Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.16
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly
-
Bos taurus ?
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg
-
Bos taurus ?
-
?
UDP-N-acetyl-D-galactosamine + apomucin bovine apomucin Bos taurus UDP + N-acetyl-D-galactosaminyl-apomucin
-
?
UDP-N-acetyl-D-galactosamine + polypeptide acceptors: synthetic peptides Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?
UDP-N-acetyl-D-galactosamine + polypeptide transfer of GalNAc to a serine or threonine residue on the acceptor protein Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?
UDP-N-acetyl-D-galactosamine + polypeptide post-translational, initial reaction in O-linked oligosaccharide biosynthesis Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Bos taurus